Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes
Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes
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DOI：
10.3389/fgene.2019.00646
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发表时间：
2019-07-05
影响因子：
3.7
通讯作者：
Dang, Ruihua
中科院分区：
文献类型：
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作者：
Gao, Yuan;Li, Shipeng;Dang, Ruihua
睾丸发育和精子发生受多个基因和非编码基因的严格调控。然而，长链非编码RNA（lncRNA）作为众多生物学过程中的关键调控因子，在牛睾丸性成熟过程中尚未得到系统的鉴定。在这项研究中，我们全面分析了lncRNA和mRNA表达谱的6牛睾丸出生后3天和13个月的RNA测序。共鉴定出23，735个lncRNA和22，118个mRNA，其中540个lncRNA（P值< 0.05）和3，525个mRNA（P调整< 0.05）在两个阶段之间显著差异表达（DE）。相应地，RT-qPCR分析结果显示与转录组数据具有良好的相关性。GO和KEGG富集分析表明，DE基因和DE lncRNA的靶基因在精子发生过程中富集。此外，我们构建了lncRNA-基因相互作用网络，因此，15 DE lncRNA和12顺式靶基因参与。目的基因（SPATA 16、TCF 21、ZPBP、PACRG、ATP 8B 3、COMP、ACE和OSBP 2）与牛的性成熟相关。此外，在牛Leydig细胞、Sertoli细胞和精原细胞中检测到lncRNA和顺式靶基因的表达。本研究对睾丸组织中的lncRNA和mRNA进行了鉴定和分析，提示lncRNA可能参与了睾丸发育和精子发生的调控。我们的研究结果为进一步研究牛lncRNA的生物学功能提供了新的见解。
Testis development and spermatogenesis are strictly regulated by numbers of genes and non-coding genes. However, long non-coding RNAs (lncRNAs) as key regulators in multitudinous biological processes have not been systematically identified in bovine testes during sexual maturation. In this study, we comprehensively analyzed lncRNA and mRNA expression profiling of six bovine testes at 3 days after birth and 13 months by RNA sequencing. 23,735 lncRNAs and 22,118 mRNAs were identified, in which 540 lncRNAs (P-value < 0.05) and 3,525 mRNAs (P-adjust < 0.05) were significantly differentially expressed (DE) between two stages. Correspondingly, the results of RT-qPCR analysis showed well correlation with the transcriptome data. Moreover, GO and KEGG enrichment analyses showed that DE genes and target genes of DE lncRNAs were enriched in spermatogenesis. Furthermore, we constructed lncRNA-gene interaction networks; consequently, 15 DE lncRNAs and 12 cis-target genes were involved. The target genes (SPATA16, TCF21, ZPBP, PACRG, ATP8B3, COMP, ACE, and OSBP2) were found associated with bovine sexual maturation. In addition, the expression of lncRNAs and cis-target genes was detected in bovine Leydig cells, Sertoli cells, and spermatogonia. Our study identified and analyzed lncRNAs and mRNAs in testis tissues, suggesting that lncRNAs may regulate testis development and spermatogenesis. Our findings provided new insights for further investigation of biological function in bovine lncRNA.