Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia
Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia
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DOI：
10.1016/j.parint.2015.12.005
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发表时间：
2016-06-01
影响因子：
1.9
通讯作者：
Ryan, Una
中科院分区：
文献类型：
--
作者：
Austen, Jill M.;Paparini, Andrea;Ryan, Una
短尾矮袋鼠（Setonix brachyurus）是西澳大利亚特有的一种脆弱的小型有袋动物。从三个不同地理位置的短尾矮袋鼠中采集血液样本;两个人湾，秃头岛和罗特内斯特岛。锥虫18 S核糖体RNA基因巢式PCR总检出率为57.3%（63/110），其中Two Peoples Bay、Bald Island和Rottnest Island的检出率分别为91.4%、85.3%和4.9%。对47例18 S PCR阳性标本进行系统发育分析，鉴定出2种科氏锥虫基因型，T. copemani基因型B是三个地区感染quokka人群最常见的基因型，总患病率为51.8%（24/47），而T基因型为34%。copemani基因型A（16/47）。混合型T. copemani基因A和B型感染率为14.9%（7/47）。在甘油醛-3-磷酸脱氢酶（GAPDH）位点的26个quokka分离株的系统发育分析，在很大程度上支持18 S分析，但确定了一个quokka分离株（Q4112-4117从两个人湾）的混合感染。T. copemani基因型B以前只从短尾矮袋鼠和吉尔伯特的potoroo中分离出来，而T. copemani基因型A具有广泛的宿主范围并且可能是致病性的。需要进一步的工作来确定T. copemani有袋动物种群。(C)2015爱思唯尔爱尔兰有限公司版权所有。
The quokka, Setonix brachyurus, is a vulnerable, small marsupial endemic to Western Australia. Blood samples were collected from quokkas from three different geographical locations; Two Peoples Bay, Bald Island and Rottnest Island. The overall prevalence of trypanosomes by nested PCR at the 18S ribosomal RNA gene was 573% (63/110) with prevalences of 91.4%, 853% and 4.9% respectively for Two Peoples Bay, Bald Island and Rottnest Island. Phylogenetic analysis conducted on 47 18S PCR positives identified two Trypanosoma copemani genotypes, with T. copemani genotype B, the most prevalent genotype infecting quokka populations from the three locations with an overall prevalence of 51.8% (24/47) compared to 34% for T. copemani genotype A (16/47). The overall prevalence of mixed T. copemani genotype A and B infections was 14.9% (7/47). Phylogenetic analysis of 26 quokka isolates at the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locus, largely supported the 18S analysis but identified a mixed infection in one quokka isolate (Q4112-4117 from Two Peoples Bay). T. copemani genotype B has previously only been isolated from quokkas and the Gilbert's potoroo whereas T. copemani genotype A has a wide host range and may be pathogenic. Further work is required to determine the clinical impact of T. copemani on marsupial populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.