A METHOD FOR THE HIGH-EFFICIENCY OF WATER-SOLUBLE CARBODIIMIDE-MEDIATED AMIDATION
A METHOD FOR THE HIGH-EFFICIENCY OF WATER-SOLUBLE CARBODIIMIDE-MEDIATED AMIDATION
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DOI：
10.1006/abio.1994.1144
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发表时间：
1994-04-01
影响因子：
2.9
通讯作者：
VIJAY, IK
中科院分区：
文献类型：
--
作者：
SEHGAL, D;VIJAY, IK
水溶性碳二亚胺广泛用于羧基胺的偶联。然而，在各种条件下获得的极易变和低收率一直是耦合中的一个严重问题。介绍了一种简单的方法，优化了偶联反应的各种参数，其中n -羟基琥珀酰亚胺辅助1-乙基-3-（3-二甲氨基丙基）氯代碳二亚胺催化酰胺化反应。产品收率通常可达90%。(C) 1994学术出版社，Inc.
Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysuccinimide is included to assist the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction is described. A product yield of up to 90% is routinely achieved. (C) 1994 Academic Press, Inc.