Individual acid aspartic proteinases (Saps) 1-6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice
Individual acid aspartic proteinases (Saps) 1-6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice
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DOI：
10.1006/mpat.2001.0478
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发表时间：
2002-02-01
影响因子：
3.8
通讯作者：
Nichterlein, T
中科院分区：
文献类型：
--
作者：
Kretschmar, M;Felk, A;Nichterlein, T
为了研究白色念珠菌个体分泌的天冬氨酸蛋白酶（Saps）是否在小鼠胃肠道感染中起作用，我们比较了SAP1-6基因的差异表达和SAP1-6蛋白的产生与SAP敲除菌株在胃肠道的侵袭和持续。利用体外表达技术（IVET），我们发现SAP4-6基因的表达率高，且在感染过程中稳步增加，SAP1-3基因的表达率偶见，且低于SAP4-6基因的表达率。采用逆转录聚合酶链反应（RT-PCR），在所有小鼠胃中均检测到SAP4和SAP6 mRNA，而在所有动物胃中均未检测到SAP2、SAP3和SAP5 mRNA，且未检测到SAP1 mRNA。此外，通过免疫电子显微镜，我们证明了Saps1-3和Saps4-6的产生，抗体与Saps1-3或Saps4-6发生交叉反应。与SAP的基因表达和产生很容易检测到的事实相反，我们无法证明与对照菌株相比，感染SAP敲除菌株的小鼠在入侵胃的能力、传播到大脑的能力、粪便脱落的持续时间和粪便中持续存在的真菌数量方面存在差异。我们得出结论，尽管产生了Saps，但个体Saps并不是小鼠胃肠道感染过程中毒力的不可或缺的因素。(C) 2002年学术出版社。
In order to investigate whether there is a role for individual secreted aspartic proteinases (Saps) of Candida albicans in gastrointestinal infection of mice we compared the differential expression of SAP1-6 genes and production of Sap1-6 proteins with invasion and persistence of SAP knockout strains in the gastrointestinal tract. Using an in vivo expression technology (IVET) we found a high percentage of expression of SAP4-6 genes which increased steadily in the course of infection, Expression of SAP1-3 genes was detected occasionally and in lower percentages than that of SAP4-6 genes. With reverse transcriptase-polymerase chain reaction (RT-PCR), mRNA for SAP4 and SAP6 were detected in the stomach of all mice, whereas SAP2, SAP3 and SAP5 mRNA were detected not in all animals and SAP1 mRNA was not detectable. Also with immunoelectron microscopy we demonstrated production of Saps1-3 as well as Saps4-6 with antibodies crossreacting with either Saps1-3 or Saps4-6. In contrast to the fact that gene expression and production of Saps were readily detectable, we were unable to demonstrate differences in the ability to invade the stomach, to disseminate to the brain as well as in the duration of faecal shedding and the number of fungi persisting in the faeces of mice infected with SAP knockout strains in comparison to control strains. We conclude that although Saps were produced, individual Saps were not indispensable factors for virulence during gastrointestinal infection of mice. (C) 2002 Academic Press.