Influence of glial growth factor and Schwann cells in a bioresorbable guidance channel on peripheral nerve regeneration
Influence of glial growth factor and Schwann cells in a bioresorbable guidance channel on peripheral nerve regeneration
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DOI：
10.1089/107632700320757
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发表时间：
2000-04-01
影响因子：
--
通讯作者：
Summerhayes, IC
中科院分区：
文献类型：
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作者：
Bryan, DJ;Holway, AH;Summerhayes, IC
使用一个建立的大鼠周围神经再生模型，我们研究了神经胶质生长因子（GGF）在神经再生中的作用，结合一种新的生物可吸收的聚（乳酸-羟基乙酸）（PLGA）的指导在体内。分离从切除的大鼠坐骨神经的1 cm段建立的雪旺细胞，并将其接种到具有或不具有GGF的神经导管上（n = 24/组）。在这些动物中重新建立了活的神经导管，并在12周的时间内评估了神经再生。组织学研究显示，与生理盐水对照相比，在外源性添加的雪旺细胞的存在下，总轴突计数和有髓鞘轴突的数量减少。相比之下，单独添加GGF增强了轴突的总数，并显着增加了血管的数量。尽管GGF与雪旺细胞的组合否定了单独使用GGF所见的轴突和血管的数量增加，但这种组合导致了最高的髓鞘形成指数和最快的传导速度。PLGA引导材料未引发任何组织学可检测的宿主反应，并且在该动物模型中允许神经再生。本研究的结果表明，该指南在体内的潜在效用，并建立了促进神经再生的GGF的作用。
Using an established rat peripheral nerve regeneration model, we investigated the role of glial growth factor (GGF) in nerve regeneration in combination with a novel bioresorbable poly(lactic-co-glycolic) acid (PLGA) guide in vivo. Schwann cells, established from a 1-cm segment of excised rat sciatic nerve, were isolated and seeded onto nerve guides with or without GGF (n = 24/group). Living nerve guides were re-established in these animals, and nerve regeneration was assessed over a period of 12 weeks. Histological studies revealed a reduction in the total axon count and the number of myelinated axons in the presence of exogenously added Schwann cells compared to saline controls. In contrast, the addition of GGF alone enhanced the total number of axons and significantly increased the number of blood vessels. Although combining GGF with Schwann cells negated the enhanced numbers of axons and blood vessels seen with GGF alone, this combination resulted in the highest myelination index and the fastest conduction velocities recorded. The PLGA guide material did not trigger any histologically detectable host response and was permissive for nerve regeneration in this animal model. The results from this study demonstrate the potential utility of this guide in vivo and establish a promotional role for GGF in nerve regeneration.