Heparan sulfate in human cutaneous Meissner's and Pacinian corpuscles
Heparan sulfate in human cutaneous Meissner's and Pacinian corpuscles
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DOI：
10.1002/ar.24328
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发表时间：
2019-12-26
影响因子：
2
通讯作者：
Vega, Jose A.
中科院分区：
文献类型：
--
作者：
Garcia-Piqueras, Jorge;Garcia-Suarez, Olivia;Vega, Jose A.
硫酸肝素蛋白聚糖是参与周围神经系统体感觉轴突引导的细胞周/细胞表面分子。然而，硫酸肝素蛋白聚糖在人皮肤感觉小体细胞外基质中的分布尚不清楚。采用两种抗硫酸肝素抗体和抗s100蛋白、抗pgp9.5、抗cd34（分别针对免疫标记基膜、雪旺细胞、轴索和帕西尼小体的中间神经内膜层）、抗IV型胶原和抗硫酸软骨素抗体，采用免疫组化和免疫荧光法确定硫酸肝素蛋白多糖在人皮肤迈斯纳小体和帕西尼小体中的定位。硫酸肝素蛋白多糖在迈斯纳氏小体中与IV型胶原共定位，位于外核片层和被囊，而不在帕西尼氏小体的内核或中间层。硫酸软骨素存在于太平洋小体的中间层，但从未与硫酸肝素蛋白聚糖共定位。本研究结果强烈提示硫酸肝素蛋白多糖与迈斯纳氏小体的板层细胞基底膜和帕西尼氏小体的复杂外核囊有关。这些结果的功能意义，如果有的话，仍有待阐明。
Heparan sulfate proteoglycans are pericellular/cell surface molecules involved in somatosensory axon guidance in the peripheral nervous system. However, the distribution of heparan sulfate proteoglycans in the extracellular matrix of human cutaneous sensory corpuscles is unknown. Immunohistochemistry and immunofluorescence assays were performed to define the localization of heparan sulfate proteoglycans in human cutaneous Meissner's and Pacinian corpuscles using two anti-heparan sulfate antibodies together with anti-S100 protein, anti-PGP9.5, anti-CD34 (to immunolabel basement membranes, Schwann cells, axon and the intermediate endoneurial layer of Pacinian corpuscles, respectively), anti-Type IV collagen, and anti-chondroitin sulfate antibodies. Heparan sulfate proteoglycans were colocalized with Type IV collagen in Meissner's corpuscles and were located in the outer core lamellae and capsule, but not in the inner core or the intermediate layer, in Pacinian corpuscles. Chondroitin sulfate was observed in the intermediate layer of Pacinian corpuscles but was never colocalized with heparan sulfate proteoglycans. The present results strongly suggest that heparan sulfate proteoglycans are associated with the basement membranes of the lamellar cells in Meissner's corpuscles and with the complex outer core capsule in Pacinian corpuscles. The functional significance of these results, if any, remains to be elucidated.