Bovine Brain Endothelial Cells Express Tight Junctions and Monoamine Oxidase Activity in Long‐Term Culture
Bovine Brain Endothelial Cells Express Tight Junctions and Monoamine Oxidase Activity in Long‐Term Culture
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长期培养中牛脑内皮细胞表达紧密连接和单胺氧化酶活性
DOI：
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发表时间：
1989
影响因子：
4.7
通讯作者：
R. Cecchelli
中科院分区：
文献类型：
--
作者：
S. Méresse;M. Dehouck;P. Delorme;M. Bensaïd;J. Tauber;C. Delbart;J. Fruchart;R. Cecchelli
Abstract: The passage of substances across the blood‐brain barrier is regulated by cerebral capillaries which possess certain distinctly different morphological and enzymatic properties compared to capillaries of other organs. Investigations of the functional characteristics of brain capillaries have been facilitated by the use of cultured brain endothelial cells, but in most studies a number of characteristics of the in vivo system are lost. To provide an in vitro system for studies of brain capillary functions, we developed a method of isolating and producing a large number of bovine brain capillary endothelial cells. These cells, absolutely free of pericyte contamination, are subcultured, at the split ratio of 1:20 (20‐fold increase of the cultured surface), with no apparent changes in cell morphology up to the fiftieth generation (10 passages). Retention of endothelial‐specific characteristics (factor V11I‐related antigen, angiotensin‐converting enzyme, and nonthrombogenic surface) is shown for brain capillary‐derived endothelial cells up to passage 10, even after frozen storage at passage 3. Furthermore, we showed that bovine brain capillary endothelial cells retain, up to the fiftieth generation, some of the characteristics of the blood‐brain barrier: occurrence of tight junctions, paucity of pinocytotic vesicles, and monoamine oxidase activity
血浆因子参与体外控制在确定培养基中生长的牛晶状体细胞的增殖。
影响因子：
3.4
作者：
Gospodarowicz,D;Massoglia,S
通讯作者：
Massoglia,S