Laser capture.
Laser capture.
复制标题
激光捕获。
DOI：
10.1007/978-1-61779-851-1_19
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Brunskill EW
中科院分区：
文献类型：
--
作者：
Potter SS;Brunskill EW
本章描述了用于离散细胞亚群的激光捕获显微切割（LCM）的详细方法。主题包括准备组织块，低温切片，处理载玻片，进行LCM，并从LCM样品中纯化RNA。笔记描述了每个操作的细节，这通常意味着成功和失败的区别。
This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.