EFFECT OF PATULIN ON MICROBICIDAL ACTIVITY OF MOUSE PERITONEAL-MACROPHAGES
EFFECT OF PATULIN ON MICROBICIDAL ACTIVITY OF MOUSE PERITONEAL-MACROPHAGES
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DOI：
10.1016/0278-6915(90)90132-7
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发表时间：
1990-01-01
影响因子：
4.3
通讯作者：
SALVAYRE, R
中科院分区：
文献类型：
--
作者：
BOURDIOL, D;ESCOULA, L;SALVAYRE, R
棒曲霉素是一种真菌代谢物，先前显示对细胞和体液免疫系统具有免疫抑制作用，研究了其对小鼠腹腔巨噬细胞某些功能的体外影响。霉菌毒素浓度为0.01-2 . μ g/ml，细胞预孵育2h。吞噬作用和吞噬体-溶酶体融合在0.1 μ以上减少。G展霉素/ml，溶酶体酶和微生物活性在0.5 μ以上。而O2的产生仅在2 μ g/ml以上受到抑制。这表明其杀伤机制不依赖于氧化爆发的产物。所使用的浓度没有降低细胞活力。在自然情况下，展青霉素可能对动物的健康构成威胁。
Patulin, a fungal metabolite shown previously to exert immunsuppresisive efects on the cellular and humoral immune systems, was examined for its in vitro effects on some functions of murine peritoneal macrophages. The cells were pre-incubated for 2 hr with mycotoxin concentrations of 0.01-2 .mu.g/ml. Phargocytosis and phagosome-lysosome fusion were diminished above 0.1 .mu.g patulin/ml and lysosomal enzymes and microbicial activity above 0.5 .mu.g/ml, whereas O2- production was inhibited only above 2 .mu.g/ml. This indicated that the killing mechanism did not depend on products of the oxidative burst. The concentrations used did not decrease the cell viability. Under natural circumstances, patulin may constitute a health risk for animals.