Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation
Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation
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DOI：
10.1159/000439165
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发表时间：
2014-01-01
影响因子：
2.7
通讯作者：
Matalova, Eva
中科院分区：
文献类型：
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作者：
Minarikova, Monika;Oralova, Veronika;Matalova, Eva
牙齿在周围的牙周组织中发育，包括牙槽骨、牙周膜和牙骨质。牙槽骨起源于涉及来自牙胚的间充质细胞的膜内骨化过程。由于大多数可用的数据与软骨内骨化有关，我们研究了牙槽骨发育的分子背景。我们研究了从小鼠下颌骨切片中分离的间充质细胞在早期牙槽骨形成阶段的成骨特性。使用PCR阵列进行基因表达的相对监测;这包括与成骨相关的84个基因的概况。为了检查牙-骨界面，选择在发育过程中具有可检测到的骨重建变化的阶段（E13.0、E14.0和E15.0）并相互比较。这些结果显示，基因Fgf 3、Ctsk、Icam-1、Mmp 9、Itga 3和Tuft 1以及多种胶原蛋白（Col 1a 2、Col 3a 1、Col 7a 1、Col 12 a1、Col 14 a1）的表达在统计学上显著增加。在Col 2a 1、Sox 9、Smad 2和Vegfb的情况下检测到表达降低。为了证实这些基因表达的变化，原位进行Mmp 9和Sox 9蛋白的免疫荧光分析。我们的研究已经确定了几个候选基因，可能是至关重要的启动牙槽骨形成，是进一步的功能研究的基础。(C)2015 S. Karger AG，巴塞尔
Teeth develop within the surrounding periodontal tissues, involving the alveolar bone, periodontal ligament and cementum. The alveolar bone originates through the process of intramembranous ossification involving mesenchymal cells from the tooth germ. As most available data are related to endochondral ossification, we examined the molecular background of alveolar bone development. We investigated the osteogenic profile of mesenchymal cells dissected from mouse mandible slices at the stage of early alveolar bone formation. Relative monitoring of gene expression was undertaken using PCR Arrays; this included the profiles of 84 genes associated with osteogenesis. To examine the tooth-bone interface, stages with detectable changes in bone remodelling during development (E13.0, E14.0 and E15.0) were chosen and compared with each other. These results showed a statistically significant increase in the expression of the genes Fgf3, Ctsk, Icam-1, Mmp9, Itga3 and Tuft1, and of a wide range of collagens (Col1a2, Col3a1, Col7a1, Col12a1, Col14a1). Decreased expression was detected in the case of Col2a1, Sox9, Smad2 and Vegfb. To confirm these changes in gene expression, immunofluorescence analyses of Mmp9 and Sox9 proteins were performed in situ. Our research has identified several candidate genes that may be crucial for the initiation of alveolar bone formation and is the basis for further functional studies. (C) 2015 S. Karger AG, Basel