QUANTITATIVE HISTOCHEMISTRY OF THE DEVELOPING HAMSTER TOOTH: ALKALINE PHOSPHATASE AND LACTIC DEHYDROGENASE
QUANTITATIVE HISTOCHEMISTRY OF THE DEVELOPING HAMSTER TOOTH: ALKALINE PHOSPHATASE AND LACTIC DEHYDROGENASE
复制标题
仓鼠牙齿发育的定量组织化学：碱性磷酸酶和乳酸脱氢酶
DOI：
--
复制
发表时间：
1961
影响因子：
3.2
通讯作者：
S. L. Bonting
中科院分区：
文献类型：
--
作者：
K. Nuki;S. L. Bonting
1. 采用显微解剖和显微化学分析的定量组织化学技术，对出生至4日龄的仓鼠上颌第一磨牙碱性磷酸酶和乳酸脱氢酶活性的定量分布进行了研究。2. 牙芽解剖区为星状网、中间层、成釉细胞、牙本质+牙釉质、成牙细胞、牙乳头3区、颈袢。3. 碱性磷酸酶分布的定量结果与Gomori染色和偶氮染色技术的结果在定性上一致，除了染色技术在低活性下给出假阴性反应。4. 碱性磷酸酶活性在离牙本质和牙釉质形成最远的区域最低，而与牙本质和牙釉质形成最密切相关的两个细胞区域活性不高；事实上，成釉细胞区是活性最低的区域之一。在形成牙本质和牙釉质基质的细胞旁边，中间层和亚成牙细胞乳突是活性最高的区域。5. 这些事实被认为是诺伊曼和诺伊曼关于钙化部位周围“屏障”中碱性磷酸酶“解毒”特性的理论的支持证据。6. 乳酸脱氢酶活性水平在所有细胞区都具有相同的数量级。离牙釉质和牙本质形成最远的区域占据了所发现范围的较低部分。
1. It has been possible to adapt the quantitative histochemical technique of microdissection and microchemical assay for the study of the quantitative distribution of alkaline phosphatase and lactic dehydrogenase activity in the first maxillary molar of the hamster, between birth and 4 days of age. 2. The zones of the tooth buds dissected out and assayed were: stellate reticulum, stratum intermedium, ameloblasts, dentine plus enamel, odontoblasts, three areas of the dental papilla, and the cervical loop. 3. The quantitative results for the alkaline phosphatase distribution were found to agree qualitatively with the results obtained by the Gomori and azo dye staining techniques, except that the staining techniques gave falsely negative reactions at low activities. 4. Alkaline phosphatase activity was lowest in the zones furthest from dentine and enamel formation, while the two zones of cells most intimately concerned in the formation of dentine and enamel did not show the highest activity; in fact the ameloblast zone was one of the lowest in activity. The highest zones of activity, stratum intermedium and sub-odontoblastic papilla, were immediately next to the cells forming the matrices of dentine and enamel. 5. These facts are considered to be supporting evidence for the theory of Neuman and Neuman regarding the "detoxifying" properties of alkaline phosphatase in a "barrier" surrounding the sites of calcification. 6. The levels of lactic dehydrogenase activity were of the same order of magnitude in all the cellular zones. The zones furthest removed from enamel and dentine formation occupied the lower part of the range found.