FINGERPRINTING G-PROTEIN-COUPLED RECEPTORS
FINGERPRINTING G-PROTEIN-COUPLED RECEPTORS
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DOI：
10.1093/protein/7.2.195
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发表时间：
1994-02-01
期刊：
影响因子：
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通讯作者：
FINDLAY, JBC
中科院分区：
文献类型：
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作者：
ATTWOOD, TK;FINDLAY, JBC
最近，我们报道了视紫红质样G蛋白偶联受体(GPCRs)识别指纹的设计。该指纹编码了7个假定的跨膜飞蛾，并对OWL复合序列数据库8.1版中的所有GPCR(共52个)进行了有效的诊断，很容易将它们与所有其他完整的膜蛋白区分开来。随着猫头鹰的体型增加了3倍，指纹已经更新，现在发现了332个与啊飞蛾匹配的受体。然而，情况变得越来越复杂：61个序列与指纹不完全匹配，总共产生了393个“匹配”。大部分的部分命中是嗅觉受体：这些受体似乎属于离散的亚家族，其中一个或多个跨膜蛾要么匹配不良，要么根本不匹配。这些结果得到了初步的系统发育分析的支持，这些分析表明嗅觉和其他各种部分匹配远离真正的命中主体。该方法为识别GPCRs提供了一个强大的诊断工具，结果与之前的观察结果一致，即信息素、cAMP和分泌素样受体属于不同的家族-这些受体具有自己独特的序列指纹，通过这些指纹可以将它们与视紫红质样超家族区分开来。
Recently we reported the design of a discriminating fingerprint for rhodopsin-like G-protein-coupled receptors (GPCRs). The fingerprint encodes the seven putative membrane-spanning moths and was potently diagnostic of all GPCRs (52 in all) in version 8.1 of the OWL composite sequence database, readily distinguishing them from all other integral membrane proteins. With a 3-fold increase in the size of OWL, the fingerprint has been updated and now finds 332 receptors that match ah the moths. The situation, however, has grown in complexity: 61 sequences make imperfect matches with the fingerprint, yielding a total of 393 'hits'. The bulk of the partial hits are olfactory receptors: these appear to fall into discrete subfamilies in which one or more of the transmembrane moths are either poorly matched or are not matched at all. These results are supported by preliminary phylogenetic analyses, which show the olfactory and various other partial matches clustering away from the main body of true hits. The approach has provided a powerful diagnostic tool for identifying GPCRs, and results are consistent with previous observations that the pheromone, cAMP and secretin-like receptors belong to separate families-these bear their own unique sequence fingerprints by which they may be distinguished from the rhodopsin-like superfamily.