Genome variability of human adenovirus type 8 causing epidemic keratoconjunctivitis during 1986-2003 in Japan
Genome variability of human adenovirus type 8 causing epidemic keratoconjunctivitis during 1986-2003 in Japan
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发表时间：
2011-11
期刊：
影响因子：
2.2
通讯作者：
X. Jin;K. Aoki;N. Kitaichi;T. Ariga;S. Ishida;S. Ohno
中科院分区：
文献类型：
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作者：
X. Jin;K. Aoki;N. Kitaichi;T. Ariga;S. Ishida;S. Ohno
目的流行性角结膜炎（EKC）是一种与社区获得性感染相关的急性接触性结膜炎。人腺病毒8型（HAdV-8）是从EKC患者中分离的主要血清型之一。DNA限制性内切酶分析进行了调查的菌株的遗传特性和它们的时间模式。方法收集1986 ~ 2003年日本EKC散发病例中分离的11株病毒，用HAdV-8型特异性抗血清中和法鉴定为HAdV-8。DNA限制性内切酶分析包括6种限制性内切酶：BamHI、HindIII、Pst I、SacI、SalI和SmaI。结果1986年HAdV-8A和HAdV-8B，1991年HAdV-8 K，1996年HAdV-8 E。HAdV-8 K是一种新的基因组类型，是用SacI酶揭示的。2003年分离的两个菌株表现出与HAdV-54相同的限制性图谱，HAdV-54于2008年描述并于2000年从日本患者中收集。结论HAdV-8的遗传变异可能存在时间上的变化。HAdV-8显示出相当大的变异性。这些变异的研究可能有助于确定HAdV感染的进化趋势和预测未来的爆发。
Purpose Epidemic keratoconjunctivitis (EKC) is a contagious acute conjunctivitis associated with community-acquired infection. Human adenovirus type 8 (HAdV-8) is one of the major serotypes isolated from patients with EKC. DNA restriction enzyme analyses were performed to investigate the genetic characteristics of the isolates and their chronological pattern. Methods Viral samples were taken from 11 strains isolated from sporadic cases of EKC and identified as HAdV-8 by the neutralization method with type-specific antiserum against HAdV-8 between 1986 and 2003 in Japan. DNA restriction enzyme analysis included six restriction enzymes: BamHI, HindIII, PstI, SacI, SalI, and SmaI. Results The restriction patterns revealed that the genome types were HAdV-8A and HAdV-8B in 1986, HAdV-8K in 1991, and HAdV-8E in 1996. HAdV-8K was a new genome type revealed with the enzyme SacI. Two strains isolated in 2003 exhibited identical restriction patterns as HAdV-54, which was described in 2008 and collected from Japanese patients in 2000. Conclusions Genetic changes might occur chronologically in HAdV-8. HAdV-8 displays considerable variability. The investigations of these variants might be helpful for defining the evolutionary tendency and to predict future outbreaks of HAdV infection.