Fungal Diagnostics
Fungal Diagnostics
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DOI：
10.1101/cshperspect.a019299
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发表时间：
2014-04-01
影响因子：
5.4
通讯作者：
Wickes, Brian
中科院分区：
文献类型：
--
作者：
Kozel, Thomas R.;Wickes, Brian
真菌感染的早期诊断是有效治疗的关键。诊断有许多障碍，如临床真菌学家数量减少，成本，结果时间以及对灵敏度和特异性的要求。此外，真菌诊断必须满足以下两种截然不同的需求：在医疗水平高的国家，与使用免疫抑制剂有关的真菌的多样性日益增加;在资源有限的国家，艾滋病患者发生大量机会性感染，因此需要进行诊断。传统的诊断方法包括临床样本的直接显微镜检查、组织病理学、培养和血清学。新兴技术包括分子诊断和临床样品中的抗原检测。使用分子和免疫分析平台的创新新技术有可能满足资源丰富和资源有限的临床环境的需求。
Early diagnosis of fungal infection is critical to effective treatment. There are many impediments to diagnosis such as a diminishing number of clinical mycologists, cost, time to result, and requirements for sensitivity and specificity. In addition, fungal diagnostics must meet the contrasting needs presented by the increasing diversity of fungi found in association with the use of immunosuppressive agents in countries with high levels of medical care and the need for diagnostics in resource-limited countries where large numbers of opportunistic infections occur in patients with AIDS. Traditional approaches to diagnosis include direct microscopic examination of clinical samples, histopathology, culture, and serology. Emerging technologies include molecular diagnostics and antigen detection in clinical samples. Innovative new technologies that use molecular and immunoassay platforms have the potential to meet the needs of both resource-rich and resource-limited clinical environments.