Salvia miltiorrhiza (Danshen) inhibits L-type calcium current and attenuates calcium transient and contractility in rat ventricular myocytes
Salvia miltiorrhiza (Danshen) inhibits L-type calcium current and attenuates calcium transient and contractility in rat ventricular myocytes
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DOI：
10.1016/j.jep.2014.10.049
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发表时间：
2014-12-02
影响因子：
5.4
通讯作者：
Chu, Li
中科院分区：
文献类型：
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作者：
Gao, Yonggang;Zhang, Kai;Chu, Li
民族药理学相关性：丹参（Salvia miltiorrhiza， SM, Danshen）是一种传统的中草药，数百年来一直被广泛用于治疗冠心病。材料和方法：我们利用全细胞膜片钳技术研究了SM对大鼠心室肌细胞l型Ca2+电流（ICa-L）的影响，并利用基于视频的边缘检测和双激发荧光光电倍增系统研究了SM对Ca2+瞬态和收缩性的影响。结果：SM溶液对ICa-L具有浓度和电压依赖性阻断作用，SM溶液剂量（10 g/l）最大抑制作用降低35.2 +/- 1.2%。然而，SM溶液并没有显著改变ICa-L的电流电压关系或逆转电位，也没有改变心脏Ca2+通道的激活和失活门控特性。同时，SM降低了心肌细胞缩短的幅度和Ca2+瞬态峰值，达到基线的90%的时间（T-r）显著降低，但达到峰值的10%的时间（T-p）没有显著延长。结论：SM显著抑制l型Ca2+通道，降低[Ca2+](i)；和成年大鼠心肌细胞的收缩性。这些发现可能与SM的心脏保护作用有关。2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Salvia miltiorrhiza (SM, Danshen), a traditional Chinese herbal drug, has been widely used for hundreds of years to treat coronary artery disease.Materials and methods: We studied the effects of SM on the L-type Ca2+ current (ICa-L) with whole-cell patch-clamp technique in rat ventricular myocytes, and its influence on Ca2+ transient and contractility using video-based edge detection and dual excitation fluorescence photomultiplier systems as well.Results: Exposure to SM solution caused a concentration- and voltage-dependent blockade of ICa-L, and the dose of SM solution (10 g/l) decreased the maximal inhibitory effect of 35.2 +/- 1.2%. However, SM solution did not significantly change the current voltage relationship or reversal potential of ICa-L, nor did it altered the activation and inactivation gating properties of cardiac Ca2+ channels. Meanwhile, SM decreased the amplitude of myocyte shortening and the peak value of Ca2+ transient with a significant decrease in the time to 90% of the baseline (T-r), but the time to 10% of the peak (T-p) was not dramatically prolonged.Conclusions: The results indicated that SM significantly inhibited L-type Ca2+ channels, decreased [Ca2+](i); and contractility in adult rat cardiac myocytes. These findings may be relevant to the cardioprotective efficacy of SM. (C) 2014 Elsevier Ireland Ltd. All rights reserved.