Application of differential display to identify genes for lung cancer detection in peripheral blood
Application of differential display to identify genes for lung cancer detection in peripheral blood
复制标题
DOI：
10.1002/ijc.10534
复制
发表时间：
2002-08-10
影响因子：
6.4
通讯作者：
Mitsuhashi, M
中科院分区：
文献类型：
--
作者：
Matsunaga, H;Hangai, N;Mitsuhashi, M
用于检测肺癌生物标志物的血液分析可以显着改善癌症患者的预后和生存率。应用扩增片段长度多态性-差异显示技术（AFLP-DD）对肺癌组织和肺癌患者外周血中的基因转录本进行鉴定。对克隆在肺癌组织、肺癌患者外周血和健康志愿者血液中的基因表达进行评估。分离的基因转录本克隆来自syndecan 1基因、collagen 1基因和2个新基因。4种转录本在正常肺组织、4种原代培养的肺细胞和6种肺癌细胞株中均有表达。从69例肺癌患者的外周血样品中分离RNA。用逆转录聚合酶链反应（RT-PCR）检测血RNA中细胞角蛋白19和4种基因mRNA转录本的存在。肺腺癌和肺鳞癌中至少有1种转录本的阳性检出率分别为79%和62%。使用RT-PCR，在53%的I期患者、100%的II期、71%的III期和81%的IV期肺癌患者中发现至少一种标记物。同时检测了20名健康志愿者的血液样本，但仅在1名患者中发现了5种转录本中的1种。这些新的分子标记物可能有助于通过从血液中分离的RNA对肺癌患者进行早期检测、分期和随访。(C)2002 Wiley-Liss，Inc.
A blood assay for detection of lung cancer biomarkers could significantly improve cancer patient prognosis and survival rates. Amplified fragment length polymorphism-differential display (AFLP-DD) was used to identify gene transcripts found in lung cancer tissue and the peripheral blood of lung cancer patients. The clones were evaluated for gene expression in lung cancer tissue, peripheral blood of lung cancer patients and healthy volunteers' blood. The isolated gene transcript clones were found to be from the syndecan 1 gene, collagen 1 gene and 2 novel genes. All 4 transcripts were expressed in normal lung tissue, 4 cultured primary lung cells and 6 lung cancer cell lines. RNA was isolated from peripheral blood samples of 69 lung cancer patients. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to test for the presence of cytokeratin 19 and the 4 gene mRNA transcripts in blood RNA. The positive detection rate of at least 1 of the 5 transcripts was 79% for lung adenocarcinoma and 62% for squamous carcinoma. Using RT-PCR, at least 1 of the markers was found in 53% of stage I patients, 100% of stage II, 71% of stage III and 81% of stage IV lung cancer patients. Blood samples from 20 healthy volunteers were also tested, but only 1 of the 5 transcripts was found in 1 patient. These new molecular markers may aid early detection, staging and follow-up of lung cancer patients by RNA isolated from blood. (C) 2002 Wiley-Liss, Inc.