Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations.
Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations.
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人类单核细胞上 HLA-DR、MB、MT 和 SB 抗原的表达：鉴定两个表型不同的单核细胞群。
DOI：
10.4049/jimmunol.133.3.1300
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发表时间：
1984
影响因子：
4.4
通讯作者：
P. Stastny
中科院分区：
文献类型：
--
作者：
Gabriel Núñez;R. Giles;Edward J. Ball;C. Hurley;J. Capra;P. Stastny
HLA-DR，SB，MB和MT抗原在不同人群的人单核细胞的表达进行了研究，使用单克隆抗体，识别不同的人IA样抗原。我们的研究结果表明，在人，如以前在其他物种中报道的，两个表型不同的群体的单核细胞或巨噬细胞可以确定的基础上表达的II类MHC抗原。几乎所有的循环单核细胞显示与HLA-DR，SB和MT相关的决定簇。此外，一个人单核细胞亚群表达MB/DS相关抗原，如MB 1、MB 3和DS框架决定簇特异性单克隆抗体所检测。大多数B淋巴细胞表达与HLA-DR相关的抗原，也存在SB 2、SB 3、MB 1、MB 3、MT 2和MT 3的特异性。静息T淋巴细胞与识别所有测试的II类MHC抗原的抗体不反应。T淋巴细胞经可溶性抗原或同种异体抗原活化后，在培养中扩增，表达DR、SB、MB和MT。贴壁细胞群中存在的大多数MB/DS+细胞为单核细胞，因为它们具有吞噬作用并具有单核细胞特异性标志物63 D3。其余的细胞没有被发现。它们可能主要包括B淋巴细胞。需要确定该子集中是否存在其他细胞，例如树突状细胞。
The expression of HLA-DR, SB, MB, and MT antigens in different populations of human mononuclear cells was investigated with the use of monoclonal antibodies that recognize distinct human Ia-like antigens. Our results indicate that in man, as previously reported in other species, two phenotypically distinct populations of monocytes or macrophages can be identified on the basis of expression of Class II MHC antigens. Virtually all circulating monocytes displayed determinants associated with HLA-DR, SB, and MT. In addition, a subpopulation of human monocytes expressed MB/DS-associated antigens, as detected with monoclonal antibodies specific for MB1, MB3, and DS-framework determinants. Most B lymphocytes expressed antigens associated with HLA-DR, and the specificities SB2, SB3, MB1, MB3, MT2, and MT3 were also present. Resting T lymphocytes were unreactive with antibodies that recognize all of the Class II MHC antigens tested. T lymphocytes activated by soluble antigen or alloantigens, and expanded in culture, expressed DR, SB, MB, and MT. The majority of the MB/DS+ cells present in the adherent population were monocytes, because they were phagocytic and had the monocyte-specific marker 63D3. The rest of the cells were not identified. They are likely to include mostly B lymphocytes. The presence of other cells, such as dendritic cells, in this subset needs to be determined.