ARRANGEMENTS OF KINETOCHORES IN MOUSE CELLS DURING MEIOSIS AND SPERMIOGENESIS
ARRANGEMENTS OF KINETOCHORES IN MOUSE CELLS DURING MEIOSIS AND SPERMIOGENESIS
复制标题
DOI：
10.1007/bf00290861
复制
发表时间：
1986-01-01
期刊：
影响因子：
1.6
通讯作者：
VALDIVIA, MM
中科院分区：
文献类型：
--
作者：
BRINKLEY, BR;BRENNER, SL;VALDIVIA, MM
用来自自身免疫性疾病硬皮病CREST患者血清的抗体研究小鼠细胞减数分裂和精子发生过程中动粒的关联和分布。粗线期细胞核的间接免疫荧光染色模式表明，每个常染色体二价体含有一个荧光点。在整个粗线期，动粒非随机排列成几个簇，分布在细胞核周围。在减数分裂前期I的后续阶段，分布是随机的，荧光点的数目从21增加到40，对应于二倍体染色体数目和在中期I定向于纺锤体极的半二价体的数目。在中期II观察到20对动粒。在精子发生过程中，着丝粒的数量与早期精子细胞的单倍体染色体数相关，但着丝粒的串联关联和聚集成一个conspicious chromocenter对应于荧光灶和中期精子细胞核的数量显着减少。在精子成熟过程中，每个细胞核的染色位点数继续减少，在成熟精子中完全没有染色。然而，从成熟精子中提取的蛋白质的免疫印迹表明，仍然存在Mr 80，000的动粒抗原。因此，成熟精子中动粒染色的缺失可能是由于染色质凝聚过程中表位的阻断。
Antibodies from the serum of patients with the autoimmune disease scleroderma CREST were used to investigate the association and distribution of kinetochores in mouse cells during meiosis and spermiogenesis. The pattern of indirect immunofluorescent staining in pachytene nuclei indicated that each autosomal bivalent contains one fluorescent spot. Throughout pachytene, the kinetochores were arranged non-randomly into several clusters and distributed around the periphery of the nucleus. In subsequent stages of meiotic prophase I, distribution was random and the number of fluorescent spots increased from 21 to 40 corresponding to the diploid chromosome number and the number of halfbivalents oriented to the spindle poles at the metaphase I. Twenty pairs of kinetochores were observed at metaphase II. During spermiogenesis, the number of kinetochores correlated with the haploid chromosome number in early spermatids but tandem association of centromeres and clustering into a conspicious chromocenter corresponded to a significant reduction in the number of fluorescent foci and mid-spermatid nuclei. The number of stained sites per nucleus continued to decrease during sperm maturation and total absence of staining was apparent in mature spermatozoa. Immunoblotting of proteins extracted from mature sperm however, indicated that a kinetochore antigen of Mr 80,000 was still present. Therefore, the absence of kinetochore staining in mature spermatozoa is probably due to the blockage of epitopes during chromatin condensation.