RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes.
RNA splicing is required to make the messenger RNA for a variant surface antigen in trypanosomes.
复制标题
需要 RNA 剪接来制造锥虫中变异表面抗原的信使 RNA。
DOI：
10.1093/nar/10.12.3591
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发表时间：
1982
影响因子：
14.9
通讯作者：
J. V. van Boom
中科院分区：
文献类型：
--
作者：
L. V. D. van der Ploeg;A. Y. Liu;P. Michels;T. de Lange;P. Borst;H. Majumder;H. Weber;G. Veeneman;J. V. van Boom
布氏锥虫中变异表面糖蛋白（VSG）118基因的表达是通过将含有该基因及其前面1- 2kb的DNA片段转座到基因组中其他地方的表达位点来激活的。通过S1核酸酶保护和RNA印迹实验，我们在这里显示合成VSG 118的锥虫中存在几种次要转录物，其中之一覆盖了整个转座片段。通过引物逆转录确定的VSG 118信使RNA（mRNA）的5'末端片段的序列与118 VSG基因的相应区域的比较表明，mRNA的5'末端34个核苷酸不是在与mRNA的其余部分邻接的118 VSG基因中编码的。我们的结论是，合成的VSG mRNA涉及剪接的一个更长的初级转录本，这可能会开始以外的转座片段。
The expression of the gene for variant surface glycoprotein (VSG) 118 in Trypanosoma brucei is activated by transposing a DNA segment containing the gene and 1-2 kb in front of it to an expression site elsewhere in the genome. By S1 nuclease protection and RNA blotting experiments we show here the presence of several minor transcripts in trypanosomes synthesizing VSG 118, one of which covers the entire transposed segment. Comparison of the sequence of the 5' terminal segment of VSG 118 messenger RNA (mRNA), determined by primed reverse transcription, and the corresponding region of the 118 VSG gene, shows that the 5' terminal 34 nucleotides of the mRNA are not encoded in the 118 VSG gene contiguous with the remainder of the mRNA. We conclude that synthesis of a VSG mRNA involves splicing of a much longer primary transcript, which may start outside the transposed segment.