Tlr0485 is a cAMP-activated c-di-GMP phosphodiesterase in a cyanobacterium Thermosynechococcus.
Tlr0485 is a cAMP-activated c-di-GMP phosphodiesterase in a cyanobacterium Thermosynechococcus.
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Tlr0485 是蓝细菌热聚球藻中的 cAMP 激活的 c-di-GMP 磷酸二酯酶。
DOI：
10.2323/jgam.2020.01.007
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
M. Ikeuchi
中科院分区：
文献类型：
--
作者：
Gen Enomoto;A. Kamiya;Yukiko Okuda;R. Narikawa;M. Ikeuchi
第二信使分子是环境信号系统的重要组成部分，用于整合多种输入并引发生理反应。在众多的第二信使中，环核苷酸cAMP和环二GMP（c-di-GMP）在细菌的环境反应中起着关键的作用。然而，这些信号系统是如何相互联系的细胞生理学的协调调节仍然难以捉摸。在嗜热蓝细菌Thermosynechococcus vulcanus菌株RKN中，蓝细菌色素光感受器感知入射光的颜色，将光信息传递到c-di-GMP的水平，c-di-GMP可能通过纤维素合酶激活诱导细胞聚集。在此，我们鉴定了由cGMP特异性磷酸二酯酶、腺苷酸环化酶和FhlA（GAF）结构域和HD-GYP结构域组成的Tlr 0485是cAMP活化的c-di-GMP磷酸二酯酶。我们还显示了生化证据表明，两个III类核苷酸环化酶，Cya 1和Cya 2，都是腺苷酸环化酶，产生cAMP在T。火山。cAMP激活的c-di-GMP磷酸二酯酶基因在蓝藻基因组中的流行表明，cAMP和c-di-GMP信号系统之间的直接串扰可能是蓝藻环境响应的关键。
Second messenger molecules are crucial components of environmental signaling systems to integrate multiple inputs and elicit physiological responses. Among various kinds of second messengers, cyclic nucleotides cAMP and cyclic di-GMP (c-di-GMP) play pivotal roles in bacterial environmental responses. However, how these signaling systems are interconnected for a concerted regulation of cellular physiology remains elusive. In a thermophilic cyanobacterium Thermosynechococcus vulcanus strain RKN, incident light color is sensed by cyanobacteriochrome photoreceptors to transduce the light information to the levels of c-di-GMP, which induces cellular aggregation probably via cellulose synthase activation. Herein, we identified that Tlr0485, which is composed of a cGMP-specific phosphodiesterases, adenylate cyclases, and FhlA (GAF) domain and an HD-GYP domain, is a cAMP-activated c-di-GMP phosphodiesterase. We also show biochemical evidence that the two class-III nucleotide cyclases, Cya1 and Cya2, are both adenylate cyclases to produce cAMP in T. vulcanus. The prevalence of cAMP-activated c-di-GMP phosphodiesterase genes in cyanobacterial genomes suggests that the direct crosstalk between cAMP and c-di-GMP signaling systems may be crucial for cyanobacterial environmental responses.
影响因子：
2.9
作者：
Miner KD;Kurtz DM Jr
通讯作者：
Kurtz DM Jr
大肠杆菌 K12 腺苷酸环化酶的纯化和表征。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yang,JK;Epstein,W
通讯作者：
Epstein,W