Phylogeography and genetic structure of Hibiscus tiliaceus -: speciation of a pantropical plant with sea-drifted seeds
Phylogeography and genetic structure of Hibiscus tiliaceus -: speciation of a pantropical plant with sea-drifted seeds
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DOI：
10.1111/j.1365-294x.2006.02963.x
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发表时间：
2006-09-01
影响因子：
4.9
通讯作者：
Tateishi, Yoichi
中科院分区：
文献类型：
--
作者：
Takayama, Koji;Kajita, Tadashi;Tateishi, Yoichi
泛热带海漂种子植物木槿的系统发育关系和空间遗传结构，及其近缘种进行了调查。这些物种的总分布范围几乎遍及全球热带地区的整个沿岸的地区，这可能是由于它们的海漂种子的传播和当地种群的经常性物种形成。利用一个c. 7500 bp的叶绿体DNA片段表明，H. tiliaceus产生了它所有的近缘种。H. tiliaceus在太平洋、大西洋和印度洋地区分布广泛且重叠。这种分布模式也证实了PCR-SSCP（聚合酶链反应扩增单链构象多态性）和PCR-SSP（PCR扩增序列特异性引物）分析超过1100个样本来自全球65个人口。F-ST统计分析和分子方差分析表明，在H.在三大洋区的tiliaceus种群。研究结果表明，由于种子的海漂，不同海区的种群之间发生了大量的基因流。观察到太平洋和大西洋种群之间的遗传差异很大，这表明该物种在两个地区之间的基因流已被阻止。H. pernambucensis和H.在大西洋地区的tiliaceus表明这两个物种在该地区之间的显着渐渗。
Phylogenetic relationships and the spatial genetic structure of a pantropical plant with sea-drifted seeds, Hibiscus tiliaceus L., and its allied species were investigated. The combined distribution range of these species is over almost the entire littoral area of the tropics worldwide, which might result from the dispersal of their sea-drifted seeds and from recurrent speciation in local populations. A phylogenetic tree constructed using the nucleotide sequences of a c. 7500-bp portion of chloroplast DNA suggested the possibility that recurrent speciation from H. tiliaceus has given rise to all of its allied species. Three major sequence haplotypes of H. tiliaceus had wide and overlapping distributions throughout the Pacific, Atlantic and Indian Ocean regions. This distribution pattern was also confirmed by PCR-SSCP (polymerase chain reaction amplification with single-strand conformation polymorphism) and PCR-SSP (PCR amplification with sequence specific primers) analyses performed on more than 1100 samples from 65 populations worldwide. Statistical analysis using F-ST and analysis of molecular variance did not show significant genetic differentiation among the H. tiliaceus populations in the three oceanic regions. The results reported here suggested substantial gene flow occurred between populations in the different oceanic regions due to sea-drifted seeds. A strong genetic difference between the Pacific and Atlantic populations of Hibiscus pernambucensis Arruda was observed, which indicates that gene flow in this species between the two regions has been prevented. The wide and dominant distribution of a haplotype shared by H. pernambucensis and H. tiliaceus in the Atlantic region suggests significant introgression between the two species in this region.