Post-Gastrectomy Osteopenia in the Rat: Bone Structure is Preserved by Retaining 10%-30% of the Oxyntic Gland Area
Post-Gastrectomy Osteopenia in the Rat: Bone Structure is Preserved by Retaining 10%-30% of the Oxyntic Gland Area
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胃切除术后%20骨质减少%20in%20the%20大鼠：%20Bone%20Structure%20is%20Preserved%20by%20Retaining%2010%-30%%20of%20the%20Oxyntic%20Gland%20Area
DOI：
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发表时间：
2002
影响因子：
1.9
通讯作者：
R. Håkanson
中科院分区：
文献类型：
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作者：
D. Lehto;D. Chen;V. Surve;R. Håkanson
背景：大鼠胃的产酸部(胃底)富含内分泌细胞，即ECL细胞和A样细胞。ECL细胞在胃泌素的控制下运作，产生组胺、嗜铬颗粒衍生的多肽胰腺抑素和一种未知的多肽激素。类A细胞产生Ghrelin，一种新发现的生长激素释放激素。外科手术切除整个腺胃(胃切除术，GX)或产酸部分(胃底切除术，FX)会导致骨量减少，这在头盖骨非常明显。我们推测，骨量减少是在手术切除眼底后发生的，因为眼底含有保存骨骼的药剂。这项研究考察了需要多少眼底才能保存正常的颅骨。方法：增加部分眼底的手术切除。测定血清胃泌素、胃泌素和胰抑素浓度。10周后处死大鼠，对颅骨进行透光分析和定量组织形态计量学研究。结果：FX使血清胃泌素水平升高，与切除眼底的数量成正比，即切除的眼底越多，血清胃泌素浓度越高。血清Ghrelin和胰腺抑素浓度与眼底切除的量成比例地降低。在接受90%或100%FX的大鼠中，颅骨显示出预期的骨丢失模式。当切除70%或更少的眼底时，未见骨丢失。结论：本研究的结果表明，需要10%-30%的基底部粘膜来保存骨。GX/FX引起的骨量减少可能是由于手术清除或减少了胃底粘膜中的一种内分泌细胞群而导致的激素缺乏。
Background: The acid-producing part of the rat stomach (fundus) is rich in endocrine cells, i.e. ECL cells and A-like cells. The ECL cells operate under gastrin control and manufacture histamine, the chromogranin-derived peptide pancreastatin and an unidentified peptide hormone. The A-like cells produce ghrelin, a newly discovered growth hormone-releasing hormone. Surgical removal of the entire glandular stomach (gastrectomy, Gx) or the acid-producing part (fundectomy, Fx) causes osteopenia, which is striking in the calvaria. We speculate that the osteopenia develops after surgical removal of the fundus, because the fundus hosts agents that preserve bone. This study examines how much of the fundus is needed to preserve normal skull bone. Methods: Increasing portions of the fundus were resected surgically. The serum gastrin, ghrelin and pancreastatin concentrations were measured. The rats were killed after 10 weeks and the calvariae were subjected to transillumination analysis and quantitative histomorphometry. Results: Fx elevated serum gastrin in proportion to the amount of fundus resected, i.e., the more fundus that was resected, the higher the serum gastrin concentration. Serum ghrelin and pancreastatin concentrations were reduced proportionally to the amount of fundus resected. In rats subjected to 90% or 100% Fx, the calvariae displayed the anticipated pattern of bone loss. No bone loss was seen when 70% or less of the fundus was resected. Conclusions: The results of the present study indicate that 10%-30% of the fundic mucosa is needed to preserve bone. The Gx/Fx-evoked osteopenia may be explained by hormonal deficiency caused by surgically eliminating or diminishing one of the endocrine cell populations in the fundic mucosa.