Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
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DOI：
10.1101/586743
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发表时间：
2019-03
影响因子：
3.5
通讯作者：
G. Johnstone;Gemma S. Cairns;B. Patton
中科院分区：
文献类型：
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作者：
G. Johnstone;Gemma S. Cairns;B. Patton
5至100 nm尺寸范围内的金刚石颗粒，称为纳米金刚石，已显示出作为光学成像的稳健荧光团的前景。我们在这里证明，由于它们的光稳定性，它们不仅适合于双光子成像，但它们允许显着的分辨率增强时，与计算超分辨率技术相结合。我们观察到的分辨率为42.5 nm时，处理双光子图像的超分辨率径向波动算法。我们显示操纵的点扩散函数的显微镜使用自适应光学。这证明了纳米金刚石的光稳定性在表征自适应光学技术或测试超分辨率或像差校正算法的弹性时也是有用的。
Particles of diamond in the 5 to 100 nm size range, known as nanodiamond, have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photo-stability, they are not only suitable for two-photon imaging, but they allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of nanodiamond can also be of use when characterising adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.