Integrative Network Analysis of the Signaling Cascades in Seedling Leaves of Bread Wheat by Large-Scale Phosphoproteomic Profiling
Integrative Network Analysis of the Signaling Cascades in Seedling Leaves of Bread Wheat by Large-Scale Phosphoproteomic Profiling
复制标题
通过大规模磷酸化蛋白质组分析对面包小麦幼叶中的信号级联进行综合网络分析
DOI：
10.1021/pr401184v
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发表时间：
2014-05-01
影响因子：
4.4
通讯作者：
Yan, Yue-Ming
中科院分区：
文献类型：
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作者：
Lv, Dong-Wen;Ge, Pei;Yan, Yue-Ming
在这里，我们通过液相色谱-串联质谱法对两个面包小麦品种进行了首次大规模叶片磷酸化蛋白质组分析。通过基因本体富集分析，总共鉴定了 1802 个明确的磷酸化位点，代表涉及各种分子功能和细胞过程的 1175 个磷蛋白。在 1175 个磷蛋白中，141 个含有 3-10 个磷酸化位点。磷酸化位点位于 N 端和 C 端区域的频率高于内部区域，并且 70% 位于保守区域之外。保守性分析表明，90.5%的磷蛋白在其他植物物种中具有磷酸化直系同源物。鉴定出十八个显着富集的磷酸化基序，其中六个是新的小麦磷酸化基序。特别是对52种磷酸化转录因子（TF）、85种蛋白激酶（PK）和16种蛋白磷酸酶（PP）进行了深入分类和分析。所有 Tyr 磷酸化位点均位于 PK 中，例如丝裂原激活 PK (MAPK) 和 SHAGGY 样激酶。构建了一个基于 PK（如 Snf1 相关激酶（SnRK）、钙依赖性 PK（CDPK）和糖原合酶激酶 3（GSK3））和 PP（包括 PP2C、PP2A 和 BRI1 抑制 1（BSU1）样蛋白（BSL））的复杂串扰磷酸化调节网络，并发现该网络可能参与快速磷酸化过程。 叶子生长。除了小麦幼苗叶片中磷酸化信号级联的潜在网络之外，我们的结果还提供了一系列磷蛋白和磷酸化位点。
Here, we conducted the first large-scale leaf phosphoproteome analysis of two bread wheat cultivars by liquid chromatography-tandem mass spectrometry. Altogether, 1802 unambiguous phosphorylation sites representing 1175 phosphoproteins implicated in various molecular functions and cellular processes were identified by gene ontology enrichment analysis. Among the 1175 phosphoproteins, 141 contained 3-10 phosphorylation sites. The phosphorylation sites were located more frequently in the N- and C-terminal regions than in internal regions, and 70% were located outside the conserved regions. Conservation analysis showed that 90.5% of the phosphoproteins had phosphorylated orthologs in other plant species. Eighteen significantly enriched phosphorylation motifs, of which six were new wheat phosphorylation motifs, were identified. In particular, 52 phosphorylated transcription factors (TFs), 85 protein kinases (PKs), and 16 protein phosphatases (PPs) were classified and analyzed in depth. All the Tyr phosphorylation sites were in PKs such as mitogen-activated PKs (MAPKs) and SHAGGY-like kinases. A complicated cross-talk phosphorylation regulatory network based on PKs such as Snf1-related kinases (SnRKs), calcium-dependent PKs (CDPKs), and glycogen synthase kinase 3 (GSK3) and PPs including PP2C, PP2A, and BRI1 suppressor 1 (BSU1)-like protein (BSL) was constructed and was found to be potentially involved in rapid leaf growth. Our results provide a series of phosphoproteins and phosphorylation sites in addition to a potential network of phosphorylation signaling cascades in wheat seedling leaves.