Disparate Candida albicans Biofilm Formation in Clinical Lipid Emulsions Due to Capric Acid-Mediated Inhibition.
Disparate Candida albicans Biofilm Formation in Clinical Lipid Emulsions Due to Capric Acid-Mediated Inhibition.
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由于癸酸介导的抑制作用，临床脂质乳剂中不同的白色念珠菌生物膜形成。
DOI：
10.1128/aac.01394-19
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发表时间：
2019
影响因子：
4.9
通讯作者：
Peters,BrianM
中科院分区：
文献类型：
--
作者：
Willems,HubertineME;Stultz,JeremyS;Coltrane,MollyE;Fortwendel,JabezP;Peters,BrianM
接受肠外营养（PN）仍然是发生真菌引起的导管相关血流感染（CR-BSI）的独立风险因素，包括多态性真菌白色念珠菌，其擅长形成耐药生物膜结构。在各种大量营养素中，PN溶液含有脂肪乳剂，可提供每日必需脂肪，通常通过中心静脉导管（CVC）输送。因此，使用体外生物膜模型系统，我们试图确定各种临床脂肪乳剂是否对C.白色念珠菌我们观察到脂肪乳剂Intralidid和Omegaven均刺激C。在基本培养基或大量营养素PN溶液中生长期间形成白色念珠菌生物膜。相反，Smoflipid抑制C.白色念珠菌生物膜形成约50%。后续研究表明，虽然Smoflipid不会损害C。对菌丝生长有明显的抑制作用。此外，当补充癸酸时，可以在Intrapidid中重现生长抑制，癸酸是Smoflipid中存在但Intrapidid中不存在的脂肪酸。癸酸也被发现剂量依赖性地抑制C。PN溶液中的白色念珠菌生物膜形成。这是第一项直接比较不同临床脂肪乳剂对C.白色念珠菌生物膜生长。本研究得出的结果需要进一步研究不同脂肪乳剂和真菌相关CR-BSI的发生率。
Receipt of parenteral nutrition (PN) remains an independent risk factor for developing catheter-related bloodstream infections (CR-BSI) caused by fungi, including by the polymorphic fungus Candida albicans, which is notoriously adept at forming drug-resistant biofilm structures. Among a variety of macronutrients, PN solutions contain lipid emulsions to supply daily essential fats and are often delivered via central venous catheters (CVCs). Therefore, using anin vitrobiofilm model system, we sought to determine whether various clinical lipid emulsions differentially impacted biofilm growth in C. albicans. We observed that the lipid emulsions Intralipid and Omegaven both stimulated C. albicans biofilm formation during growth in minimal medium or a macronutrient PN solution. Conversely, Smoflipid inhibited C. albicans biofilm formation by approximately 50%. Follow-up studies revealed that while Smoflipid did not impair C. albicans growth, it did significantly inhibit hypha formation and hyphal elongation. Moreover, growth inhibition could be recapitulated in Intralipid when supplemented with capric acid—a fatty acid present in Smoflipid but absent in Intralipid. Capric acid was also found to dose dependently inhibit C. albicans biofilm formation in PN solutions. This is the first study to directly compare different clinical lipid emulsions for their capacity to affect C. albicans biofilm growth. Results derived from this study necessitate further research regarding different lipid emulsions and rates of fungus-associated CR-BSIs.