NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PROCYANIDINS FROM CACAO BEANS AND GRAPE SEEDS
NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PROCYANIDINS FROM CACAO BEANS AND GRAPE SEEDS
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DOI：
10.1016/0021-9673(93)83368-3
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发表时间：
1993-11-19
影响因子：
4.1
通讯作者：
CHEYNIER, V
中科院分区：
文献类型：
--
作者：
RIGAUD, J;ESCRIBANOBAILON, MT;CHEYNIER, V
采用正相硅胶柱和二氯甲烷-甲醇-甲酸-水混合物梯度洗脱液的HPLC方法，开发分离原花青素的分子质量的基础上，没有衍生化。该方法已成功地应用于可可豆和葡萄籽中原花青素提取物的分析。单体和主要二聚体被解析为离散峰。低聚物和聚合物组分以聚合度增加的顺序洗脱，如通过测定乙酰化后使用凝胶渗透色谱法从正相柱收集的连续级分的平均分子量所证实的。
An HPLC method using a normal-phase silica column and a gradient of dichloromethane-methanol-formic acid-water mixtures as the eluent was developed to separate procyanidins on a molecular mass basis, without derivatization. It was successfully applied to the analysis of procyanidin extracts from cacao beans and grape seeds. The monomers and major dimers were resolved as discrete peaks. Oligomeric and polymeric components were eluted in order of increasing degree of polymerization, as confirmed by determining the average molecular mass of successive fractions collected from the normal-phase column using gel permeation chromatography, after acetylation.