SCANNING ELECTRON-MICROSCOPY OF DROSOPHILA EMBRYOGENESIS .1. STRUCTURE OF EGG ENVELOPES AND FORMATION OF CELLULAR BLASTODERM
SCANNING ELECTRON-MICROSCOPY OF DROSOPHILA EMBRYOGENESIS .1. STRUCTURE OF EGG ENVELOPES AND FORMATION OF CELLULAR BLASTODERM
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DOI：
10.1016/0012-1606(76)90070-1
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发表时间：
1976-01-01
影响因子：
2.7
通讯作者：
MAHOWALD, AP
中科院分区：
文献类型：
--
作者：
TURNER, FR;MAHOWALD, AP
作为对果蝇胚胎发生的一系列详细观察的一部分，我们用S[扫描]EM研究了原肠形成前卵的保护性覆盖物和胚胎的表面变化。四个专业化的绒毛膜被描述：腹，珠孔锥，盖，和后增厚。除去保护层后，可以观察到显影过程中的表面变化。在前8次同步核分裂期间，密集的薄微突起阵列覆盖整个胚胎。第9次分裂后，373-408个细胞核到达表面，并位于细胞质突起中。从合胞体胚基阶段表面隆起的数量计数，确定发生13次同步分裂，产生5600-6500个表面核。在细胞胚盘的形成过程中，潜在细胞的位置被来自每个细胞的密集微突起图案所掩盖。随着胚盘的完成，细胞表面变得光滑，细胞轮廓清晰。SEM在发展研究中的有用性进行了讨论。
As part of a series of detailed observations on embryogenesis in Drosophila, the protective coverings of the egg and surface changes in the embryo prior to gastrulation were studied with the S[scanning]EM. Four specializations of the chorion are described: the plastron, micropylar cone, operculum, and the posterior thickening. After removal of the protective coverings the surface changes during development can be observed. During the first 8 synchronous nuclear divisions a dense array of thin microprojections covers the whole embryo. After the 9th division 373-408 nuclei reach the surface and become located in cytoplasmic projections. From counts of the number of surface bulges during the syncytial blastema stages, it was established that 13 synchronous divisions take place producing 5600-6500 surface nuclei. During formation of the cellular blastoderm, the location of the prospective cells becomes obscured by a dense pattern of microprojections from each cell. With the completion of the blastoderm, the surfaces of the cells become smooth and the cell outlines distinct. The usefulness of the SEM in developmental studies is discussed.