Alteration of bFGF expression with growth and age in rat molar periodontal ligament
Alteration of bFGF expression with growth and age in rat molar periodontal ligament
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DOI：
10.2319/011910-38.1
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发表时间：
2010-09-01
影响因子：
3.4
通讯作者：
Hosomichi, Jun
中科院分区：
文献类型：
--
作者：
Sako, Emi;Hosomichi, Jun
目的：验证碱性成纤维细胞生长因子免疫组织化学变化不随年龄变化的假说。材料与方法：30只雄性Wistar-ST大鼠随机分为生长期(5、9、15周龄)和增龄组(6、12、18月龄)。在上颌第一磨牙远中颊根部平行切取5µm厚的连续矢状面切片。石蜡包埋组织切片，苏木精-伊红染色，兔抗碱性成纤维细胞生长因子多克隆抗体染色。结果：上颌第一磨牙牙周膜碱性成纤维细胞生长因子免疫反应阳性细胞的数量和面积随年龄增长而减少。牙根分叉区碱性成纤维细胞生长因子免疫反应细胞数量较多，受咬合力影响最大。无论年龄大小，牙周膜中碱性成纤维细胞生长因子的产生都可能受到功能需求的影响。碱性成纤维细胞生长因子在牙周膜中的表达随年龄增长而减少。(角度直角。2010；80：904-911。)
Objective: To test the hypothesis that immunohistochemical changes in expression of basic fibroblast growth factor in the periodontal ligament do not change with age.Materials and Methods: Thirty male Wistar-ST rats were divided into growing groups (5, 9, and 15 weeks of age) and aging groups (6, 12, and 18 months of age). Serial sagittal sections (5 mu m thick) were cut parallel at the distobuccal roots of the maxillary first molar. Paraffin-embedded tissue sections were stained with hematoxylin and eosin and rabbit polyclonal antibodies to basic fibroblast growth factor.Results: The number and the area of basic fibroblast growth factor immunoreactive cells in the periodontal ligament of the maxillary first molar decreased with age. The number of basic fibroblast growth factor immunoreactive cells was much greater in the root furcation area, which experiences the greatest effect of occlusal force. Regardless of age, the production of basic fibroblast growth factor in the periodontal ligament may occur subject to functional demand.Conclusions: The hypothesis is rejected. The expression of basic fibroblast growth factor in the periodontal ligament decreased with age. (Angle Orthod. 2010;80:904-911.)