Fine physical mapping of the rice stripe resistance gene locus, Stvb-i
Fine physical mapping of the rice stripe resistance gene locus, Stvb-i
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DOI：
10.1007/s001220051449
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发表时间：
2000-07-01
影响因子：
5.4
通讯作者：
Iwasaki, M
中科院分区：
文献类型：
--
作者：
Hayano-Saito, Y;Saito, K;Iwasaki, M
Stvb-i 基因赋予水稻条纹病抗性。对于定位克隆，我们构建了一个物理图谱，其侧翼标记之间的距离为 1.8 cM，由 18 个细菌人工染色体 (BAC) 克隆组成，位于水稻 11 号染色体上的 Stvb-i 位点周围。这 18 个克隆是通过筛选来自粳稻品种 Shimokita 的 BAC 文库来分离的，其中包含三个 Stvb-i 连接的 RFLP 标记和酵母人工染色体的 DraI 消化的 DNA （YAC）克隆。 Southern杂交和限制性内切酶分析的结果表明这些BAC克隆是连续的并且覆盖了含有Stvb-i等位基因的约700kb区域。利用 BAC 插入 DNA 的末端和内部片段，在包括 Stvb-i 基因座在内的一个小染色体区域内生成了 33 个分子标记。使用这些标记对抗性品种和从 120 个 F-2 个体中选出的四个附近重组体进行基因分型分析表明，Stvb-i 包含在一个大约 286 kb 的区域内，该区域覆盖有两个重叠的 BAC 克隆。
The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F-2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones.