Eukaryotic DNA diverges at a long and complex pyrimidine.purine tract that can adopt altered conformations.
Eukaryotic DNA diverges at a long and complex pyrimidine.purine tract that can adopt altered conformations.
复制标题
真核DNA在长而复杂的嘧啶-嘌呤束处发生分歧，可以采用改变的构象。
DOI：
--
复制
发表时间：
1986
影响因子：
4.8
通讯作者：
D. M. Skinner
中科院分区：
文献类型：
--
作者：
R. Fowler;D. M. Skinner
百慕大地蟹的一个复杂卫星DNA的三个克隆重复单元之间的结构域显示出主要的序列分歧，其中包含一个重复的多嘧啶。多嘌呤段，包括在CCT两组间嵌入的长脑脊液束。AGG和CGCAC。GTGCG及其变体。结构域采用至少两种类型的改变构象，它们明显受pH值和负超螺旋密度的影响；只有一种对离子强度敏感。螺旋结构中与超线圈相关的扭曲在该区域的成分偏置中断点和附近类似但不那么广泛的发散区域最为明显。由于该结构域是单个卫星重复单元之间主要序列分歧的位点，因此改变的构象可能涉及重复单元之间的位点特异性重组，无论是串联排列的重复单元还是分散在整个基因组中的重复单元。
A domain exhibiting major sequence divergences among three cloned repeat units of a complex satellite DNA of the Bermuda land crab contains a repetitive polypyrimidine.polypurine segment consisting of a long C.G tract embedded between runs of CCT.AGG and CGCAC.GTGCG and their variations. The domain adopts at least two types of altered conformations that are markedly affected by pH and negative superhelical density; only one is sensitive to ionic strength. Supercoil-dependent distortions in helical structure are most pronounced at points of interruption in compositional bias in this domain and a similar although less extensive, divergent domain nearby. Since the domain is the site of major sequence divergences among individual satellite repeat units, the altered conformations may be involved in site-specific recombination between repeat units, either those arranged in tandem or those scattered throughout the genome.