Isotope enhanced approaches in metabolomics.
Isotope enhanced approaches in metabolomics.
复制标题
代谢组学中的同位素增强方法。
DOI：
10.1007/978-94-007-4954-2_8
复制
发表时间：
2012
影响因子：
--
通讯作者：
Raftery,Daniel
中科院分区：
文献类型：
--
作者：
Gowda,GANagana;Shanaiah,Narasimhamurthy;Raftery,Daniel
快速发展的“代谢组学”领域，其中从体液、细胞或组织中定量检测大量代谢物，在一个步骤中，为许多学科带来了巨大的潜力，包括早期疾病诊断、治疗监测、系统生物学、药物发现和营养科学。由于其能够在完整的生物样品中重复和定量地检测大量代谢物，核磁共振（NMR）光谱已成为代谢组学中最强大的分析技术之一。利用2H、13C、15N和31P等核素对生物样品进行同位素标记，无论是在体内还是在体内，都大大提高了我们识别低浓度代谢物和追踪重要代谢途径的能力。考虑到生物样品的NMR光谱的有限灵敏度和高复杂性，已经做出努力通过同位素标记方法来增加灵敏度和选择性，这为解开生物复杂性和了解健康和各种疾病状况中的细胞功能铺平了新的途径。本章介绍了目前的发展，同位素标记的代谢物在体内以及asex体内，和他们潜在的代谢组学应用。
The rapidly growing area of “metabolomics,” in which a large number of metabolites from body fluids, cells or tissue are detected quantitatively, in a single step, promises immense potential for a number of disciplines including early disease diagnosis, therapy monitoring, systems biology, drug discovery and nutritional science. Because of its ability to detect a large number of metabolites in intact biological samples reproducibly and quantitatively, nuclear magnetic resonance (NMR) spectroscopy has emerged as one of the most powerful analytical techniques in metabolomics. NMR spectroscopy of biological samples with isotope labeling of metabolites using nuclei such as2H,13C,15N and31P, eitherin vivoorex vivo,has dramatically improved our ability to identify low concentrated metabolites and trace important metabolic pathways. Considering the somewhat limited sensitivity and high complexity of NMR spectra of biological samples, efforts have been made to increase sensitivity and selectivity through isotope labeling methods, which pave novel avenues to unravel biological complexity and understand cellular functions in health and various disease conditions. This chapter describes current developments in isotope labeling of metabolitesin vivoas well asex vivo,and their potential metabolomics applications.