Phylotype diversity of deep-sea hydrothermal vent prokaryotes trapped by 0.2- and 0.1-μm-pore-size filters

Phylotype diversity of deep-sea hydrothermal vent prokaryotes trapped by 0.2- and 0.1-μm-pore-size filters
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DOI:
10.1007/s00792-007-0070-5
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发表时间:
2007-07-01
期刊:
影响因子:
2.9
通讯作者:
Kimura, Hiroyuki
Kimura, Hiroyuki
中科院分区:
生物学3区
文献类型:
--
作者:
Naganuma, Takeshi;Miyoshi, Tatsuo;Kimura, Hiroyuki

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利用0.2和0.1 μ m孔径过滤器捕获的708个热液喷口原核生物克隆,共构建了11个16S rRNA基因文库,包括34个古细菌和72个细菌种型。古菌群和细菌群分别以绿古菌群和变形菌群为主。新的无亲缘关系的种型仅出现在0.1 μ m捕获种群中。
Eleven 16S rRNA gene clone libraries including 34 archaeal and 72 bacterial phylotypes were constructed from total 708 clones of hydrothermal vent prokaryotes trapped by 0.2- and 0.1-mu m-pore-size filters. Crenarchaeota and Proteobacteria phylotypes dominated the archaeal and bacterial populations, respectively. Novel unaffiliated phylotypes occurred only in the 0.1-mu m-trapped populations.