Rice Proteome Database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003
Rice Proteome Database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003
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DOI：
10.1093/nar/gkh020
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发表时间：
2004-01-01
影响因子：
14.9
通讯作者：
Higo, K
中科院分区：
文献类型：
--
作者：
Komatsu, S;Kojima, K;Higo, K
水稻蛋白质组数据库是第一个描述水稻蛋白质组的详细数据库。当前版本包含 21 个基于水稻组织和亚细胞区室蛋白质二维聚丙烯酰胺凝胶电泳 (2D-PAGE) 的参考图谱。这些参考图谱包含 11,941 个已识别的蛋白质，显示组织和亚细胞定位，对应于数据库中的 4180 个单独的蛋白质条目。水稻蛋白质组数据库包含每种蛋白质的计算属性，例如分子量、等电点和表达；通过实验确定的特性，例如使用蛋白质测序仪和质谱获得的氨基酸序列；以及数据库搜索的结果，例如序列同源性。该数据库可通过关键词、登录号、蛋白质名称、等电点、分子量和氨基酸序列进行搜索，或者通过在 2D-PAGE 参考图谱之一上选择一个点进行搜索。为蛋白质组学工具和其他 2D-PAGE 数据库提供交叉引用，从而提供与其他分子数据库的许多链接。水稻蛋白质组数据库中的信息每周更新一次，可在万维网上获取：http://gene64.dna.affrc.go.jp/RPD/。
The Rice Proteome Database is the first detailed database to describe the proteome of rice. The current release contains 21 reference maps based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of proteins from rice tissues and subcellular compartments. These reference maps comprise 11 941 identified proteins showing tissue and subcellular localization, corresponding to 4180 separate protein entries in the database. The Rice Proteome Database contains the calculated properties of each protein such as molecular weight, isoelectric point and expression; experimentally determined properties such as amino acid sequences obtained using protein sequencers and mass spectrometry; and the results of database searches such as sequence homologies. The database is searchable by keyword, accession number, protein name, isoelectric point, molecular weight and amino acid sequence, or by selection of a spot on one of the 2D-PAGE reference maps. Cross-references are provided to tools for proteomics and to other 2D-PAGE databases, which in turn provide many links to other molecular databases. The information in the Rice Proteome Database is updated weekly, and is available on the World Wide Web at http://gene64.dna.affrc.go.jp/RPD/.