P2X5 receptors are expressed on neurons containing arginine vasopressin and nitric oxide synthase in the rat hypothalamus
P2X5 receptors are expressed on neurons containing arginine vasopressin and nitric oxide synthase in the rat hypothalamus
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DOI：
10.1016/j.brainres.2006.04.126
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发表时间：
2006-07
期刊：
影响因子：
2.9
通讯作者：
Z. Xiang;Cheng He;G. Burnstock
中科院分区：
文献类型：
--
作者：
Z. Xiang;Cheng He;G. Burnstock
本研究采用单标和双标免疫荧光及逆转录聚合酶链式反应(RT-PCR)方法，发现大鼠下丘脑中广泛分布着P2X5受体。在下丘脑的神经元中，P2X5受体表达最高的区域是脑室旁核和视上核。在腹内侧核的神经元中，P2X5免疫荧光强度较低。70%~90%的室旁核神经元和46%~58%的视上核和副神经分泌核团的神经元与精氨酸加压素(AVP)共存。在视交叉上核和腹内侧核，没有一个表达P2X5受体的神经元与AVP共存。87-90%的侧脑室旁核神经元和42-56%的副神经分泌核团、视上核和腹内侧核的神经元与神经元型一氧化氮合酶(NNOS)共存。视交叉上核内表达P2X5受体的神经元均未与nNOS共定位。这些发现为嘌呤能和硝能或加压素能神经递质系统之间可能的功能相互作用提供了形态学基础。
In this study, the P2X5receptor was found to be distributed widely in the rat hypothalamus using single and double labeling immunofluorescence and reverse transcriptase-polymerase chain reaction (RT-PCR) methods. The regions of the hypothalamus with the highest expression of P2X5receptors in neurons are the paraventricular and supraoptic nuclei. The intensity of P2X5immunofluorescence in neurons of the ventromedial nucleus was low. 70–90% of the neurons in the paraventricular nucleus and 46–58% of neurons in the supraoptic and accessory neurosecretory nuclei show colocalization of P2X5receptors and arginine vasopressin (AVP). None of the neurons expressing P2X5receptors shows colocalization with AVP in the suprachiasmatic and ventromedial nuclei. 87–90% of the neurons in the lateral and ventral paraventricular nucleus and 42–56% of the neurons in the accessory neurosecretory, supraoptic and ventromedial nuclei show colocalization of P2X5receptors with neuronal nitric oxide synthase (nNOS). None of the neurons expressing P2X5receptors in the suprachiasmatic nucleus shows colocalization with nNOS. These findings provide a morphological basis for possible functional interactions between the purinergic and nitrergic or vasopressinergic neurotransmitter systems.