PhytophthoraSpecies Associated with Roots of Native and Non-native Trees in Natural and Managed Forests
PhytophthoraSpecies Associated with Roots of Native and Non-native Trees in Natural and Managed Forests
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DOI：
10.1007/s00248-020-01563-0
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发表时间：
2020-08-02
影响因子：
3.6
通讯作者：
Burgess, Treena, I
中科院分区：
文献类型：
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作者：
Bose, Tanay;Wingfield, Michael J.;Burgess, Treena, I
根作为一个生物过滤器，只允许一部分土壤相关的微生物多样性感染植物。微生物多样性包括对植物有益和有害的微生物，其中植锈菌是引起各种土传植物病害的最重要的有害微生物类群之一。我们使用了一个元条形码的方法与Phytophthora特异性引物比较的多样性和丰富度ofPhytophthoraspecies与本地和非本地树木的根，使用不同类型的土壤接种物收集的本地和管理的森林。具体而言，我们分析了（1）两个非本地树种（巨桉和黑荆树）和本地树木的根，（2）两个非本地树种的根，从体内植物诱饵试验，（3）从外地收集的根与那些从诱饵试验，和（4）从外地收集的根和土壤样品。土壤来源和根土相互作用显著影响植物类物种丰富度。此外，物种丰富度和群落组成显着不同的田间根样品和田间土壤样品之间的Phytophthorasspecies在土壤中的数量比在根。结果还揭示了南非Phytophthoraspecies的大量和以前未发现的多样性。
Roots act as a biological filter that exclusively allows only a portion of the soil-associated microbial diversity to infect the plant. This microbial diversity includes organisms both beneficial and detrimental to plants.Phytophthoraspecies are among the most important groups of detrimental microbes that cause various soil-borne plant diseases. We used a metabarcoding approach withPhytophthora-specific primers to compare the diversity and richness ofPhytophthoraspecies associated with roots of native and non-native trees, using different types of soil inocula collected from native and managed forests. Specifically, we analysed (1) roots of two non-native tree species (Eucalyptus grandisandAcacia mearnsii) and native trees, (2) roots of two non-native tree species from an in vivo plant baiting trial, (3) roots collected from the field versus those from the baiting trial, and (4) roots and soil samples collected from the field. The origin of the soil and the interaction between root and soil significantly influencedPhytophthoraspecies richness. Moreover, species richness and community composition were significantly different between the field root samples and field soil samples with a higher number ofPhytophthoraspecies in the soil than in the roots. The results also revealed a substantial and previously undetected diversity ofPhytophthoraspecies from South Africa.