Thrombin receptor overexpression in malignant and physiological invasion processes
Thrombin receptor overexpression in malignant and physiological invasion processes
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DOI：
10.1038/nm0898-909
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发表时间：
1998-08-01
期刊：
影响因子：
82.9
通讯作者：
Bar-Shavit, R
中科院分区：
文献类型：
--
作者：
Even-Ram, S;Uziely, B;Bar-Shavit, R
虽然可溶性和基质固定化蛋白酶参与肿瘤细胞的侵袭和转移是公认的，蛋白水解激活的细胞表面受体的作用还没有得到阐明。我们在这里报告，凝血酶受体，蛋白酶激活受体家族的成员，优先表达在高转移性人乳腺癌细胞系和乳腺癌活检标本。凝血酶受体反义cDNA的引入显著抑制了转移性乳腺癌细胞通过重建基底膜的侵袭。在人胚胎胎盘着床期间，凝血酶受体在侵入的细胞滋养层细胞中短暂表达。这些结果强调凝血酶受体参与与肿瘤进展和正常胚胎发育相关的细胞侵袭。
Although the involvement of soluble and matrix-immobilized proteases in tumor cell invasion and metastasis is well recognized, the role of proteolytically activated cell surface receptors has not been elucidated. We report here that thrombin receptor, a member of the protease-activated receptor family, is preferentially expressed in highly metastatic human breast carcinoma cell lines and breast carcinoma biopsy specimens. Introduction of thrombin receptor antisense cDNA considerably inhibited the invasion of metastatic breast carcinoma cells in culture through a reconstituted basement membrane. During placental implantation of the human embryo, thrombin receptor is transiently expressed in the invading cytotrophoblasts. These results emphasize the involvement of thrombin receptor in cell invasion associated with tumor progression and normal embryonic development.