CRYSTAL PROTEIN INTERACTIONS STUDIED BY OVERGROWTH OF CALCITE ON BIOGENIC SKELETAL ELEMENTS
CRYSTAL PROTEIN INTERACTIONS STUDIED BY OVERGROWTH OF CALCITE ON BIOGENIC SKELETAL ELEMENTS
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DOI：
10.1016/0022-0248(94)90283-6
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发表时间：
1994-09-01
影响因子：
1.8
通讯作者：
ADDADI, L
中科院分区：
文献类型：
--
作者：
AIZENBERG, J;ALBECK, S;ADDADI, L
晶体形成生物控制的一个关键参数是一组酸性大分子与矿物相的相互作用。在这里，我们利用合成方解石晶体的外延过度生长来研究蛋白质-方解石相互作用，其中闭塞大分子从生物基质中局部释放。大分子随后与新形成的过度生长的晶体相互作用，导致方解石形态发生改变。这种新颖的方法提供了一种在对酸性大分子构象状态影响最小的条件下绘制晶体-蛋白质相互作用的方法。我们表明，从方解石海绵针和软体动物棱镜释放的蛋白质与方解石的{001}和{01l}面特异性相互作用，而从棘皮动物骨骼元素释放的蛋白质仅与{01l}面相互作用。即使在同一生物体和同一元件内，过度生长的晶体受蛋白质影响的程度也会有所不同，具体取决于骨架元件的位点和晶体取向。
A key parameter in the biological control of crystal formation is the interaction of a group of acidic macromolecules with the mineral phase. Here we study protein-calcite interactions using epitaxial overgrowth of synthetic calcite crystals under conditions in which local release of occluded macromolecules from the biogenic substrate occurs. The macromolecules subsequently interact with the newly formed overgrown crystals, resulting in modified calcite morphology. This novel method provides a means of mapping crystal-protein interactions under conditions that minimally affect the conformational states of the acidic macromolecules. We show that proteins released from calcitic sponge spicules and mollusc prisms specifically interact with {001} and {01l} faces of calcite, whereas proteins released from echinoderm skeletal elements only interact with {01l} faces. The extent to which the overgrown crystals are affected by the proteins varies even in the same organism and within the same element, depending on the site and crystallographic orientation of the skeletal elements.