The entry of Theileria parva sporozoites into bovine lymphocytes: evidence for MHC class I involvement.
The entry of Theileria parva sporozoites into bovine lymphocytes: evidence for MHC class I involvement.
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DOI：
10.1083/jcb.113.1.87
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发表时间：
1991-04
期刊：
影响因子：
--
通讯作者：
Musoke AJ
中科院分区：
文献类型：
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作者：
Shaw MK;Tilney LG;Musoke AJ
我们已经研究了小泰勒虫孢子进入易感牛淋巴细胞的过程，并已开始确定参与该过程的可能的分子相互作用之一。进入过程涉及一系列确定的事件，我们已经使用了一些实验程序结合定量的方法来检查这个过程的各个方面。T.细小孢子是不运动的生物体，最初的子孢子-淋巴细胞相互作用是偶然事件，可在0-2 ℃下发生。该过程中的所有后续阶段都是温度依赖性的，需要活的完整子孢子和宿主细胞的参与，并涉及宿主细胞表面膜或细胞骨架的一些细胞松弛素可降解的重排。与主要组织相容性复合体（MHC）I类分子（IL-A 19，IL-A 88）和β 2微球蛋白（B1 G6）反应的抗体（mAb）可抑制子孢子进入，而与MHC II类分子（IL-A 21，J 11）和常见的泛白细胞表面抗原（IL-A 87; CD 11 a的牛等效物）反应的mAb则无作用。这些结果表明，MHC I类分子在T.细小子孢子进入牛淋巴细胞，尽管精确的作用还没有确定。一旦在淋巴细胞内内化，该过程在37 ° C下需要不到3分钟，子孢子迅速从包封宿主细胞膜逃逸;该过程与子孢子棒状体和微球的内容物的排出同时发生。胞质内寄生虫被一层子孢子衍生的模糊物质覆盖，宿主细胞微管迅速与之结合。
We have examined the process of Theileria parva sporozoite entry into susceptible bovine lymphocytes and have begun to identify one of the possible molecular interactions involved in the process. The entry process involves a defined series of events and we have used a number of experimental procedures in combination with a method of quantitation to examine various aspects of this process. T. parva sporozoites are nonmotile organisms and the initial sporozoite-lymphocyte interaction is a chance event which can occur at 0-2 degrees C. All subsequent stages in the process are temperature dependent, require the participation of live intact sporozoites and host cells, and involve some cytochalasin-inhibitable rearrangement of the host cell surface membrane or cytoskeleton. Sporozoite entry can be inhibited by antibodies (mAbs) reactive with major histocompatibility complex (MHC) class I molecules (IL-A 19, IL-A 88) and with beta 2 microglobulin (B1G6), whereas mAbs reactive with MHC class II molecules (IL-A 21, J 11), and a common panleucocyte surface antigen, (IL-A 87; a bovine equivalent of CD 11a) have no effect. These results indicate that MHC class I molecules play a role in the process of T. parva sporozoite entry into bovine lymphocytes although as yet the precise role has not been determined. Once internalized within the lymphocyte, a process that takes less than 3 min at 37 degrees C, the sporozoite rapidly escapes from the encapsulating host cell membrane; a process which occurs concurrently with the discharge of the contents of the sporozoite rhoptries and microspheres. The intracytoplasmic parasite is covered by a layer of sporozoite-derived fuzzy material to which host cell microtubules rapidly become associated.