Microfluidic co-culture of epithelial cells and bacteria for investigating soluble signal-mediated interactions.
Microfluidic co-culture of epithelial cells and bacteria for investigating soluble signal-mediated interactions.
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DOI：
10.3791/1749
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发表时间：
2010-04
期刊：
影响因子：
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通讯作者：
Jeongyun Kim;M. Hegde;A. Jayaraman
中科院分区：
文献类型：
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作者：
Jeongyun Kim;M. Hegde;A. Jayaraman
人类胃肠道是肠道上皮细胞和非致病性细菌共存的独特环境。有人提出，病原体在皮层中遇到的微环境在决定定殖程度方面很重要。目前用于研究病原体定殖的培养方法不太适合于研究这种假设，因为它们不能以模拟胃肠道微环境的方式共培养细菌和上皮细胞。在这里，我们描述了一个微流控共培养模型，使真核细胞和细菌的独立培养，并测试病原体定植的微环境的影响。共培养模型通过在HeLa细胞之间形成一个细菌性大肠杆菌生物膜，然后引入肠出血性大肠杆菌来证明。大肠杆菌（EHEC）进入肠道，其顺序模拟胃肠道感染的事件顺序。
The human gastrointestinal (GI) tract is a unique environment in which intestinal epithelial cells and non-pathogenic (commensal) bacteria coexist. It has been proposed that the microenvironment that the pathogen encounters in the commensal layer is important in determining the extent of colonization. Current culture methods for investigating pathogen colonization are not well suited for investigating this hypothesis as they do not enable co-culture of bacteria and epithelial cells in a manner that mimics the GI tract microenvironment. Here we describe a microfluidic co-culture model that enables independent culture of eukaryotic cells and bacteria, and testing the effect of the commensal microenvironment on pathogen colonization. The co-culture model is demonstrated by developing a commensal Escherichia coli biofilm among HeLa cells, followed by introduction of enterohemorrhagic E. coli (EHEC) into the commensal island, in a sequence that mimics the sequence of events in GI tract infection.