Membrane protein variations associated with in vitro passage of Borrelia burgdorferi.
Membrane protein variations associated with in vitro passage of Borrelia burgdorferi.
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与伯氏疏螺旋体体外传代相关的膜蛋白变异。
DOI：
10.1128/iai.64.2.392-398.1996
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发表时间：
1996
影响因子：
3.1
通讯作者：
Gherardini,FC
中科院分区：
文献类型：
--
作者：
Carroll,JA;Gherardini,FC
莱姆病的病原体伯氏疏螺旋体在体外反复传代会丧失毒力。定义转化为无毒后发生的变化可能有助于确定毒力因子和发病机制。我们已经使用了交叉吸附技术和二维非平衡pH梯度电泳来比较B的毒性（低传代）和无毒（高传代）变体。Burgdorferi B31.使用交叉吸附的兔血清探针免疫印迹，我们确定了10个低通道相关的蛋白质（相对分子质量为78，58，49，34，33，28，24，20，和16 kDa）独特的强毒株B31。交叉吸附的人血清检测到5个蛋白质的大小相似（78，58，34，28，和20 kDa），这表明这些蛋白质中的几个在人类感染过程中表达。通过探测内膜和外膜，观察到两种特异性定位于外膜的蛋白质（58和33 kDa）。一个额外的低通道相关的蛋白质（28 kDa）被确定时，从低和高压菌株B31的变种外膜进行了比较，通过二维非平衡pH梯度电泳。
Borrelia burgdorferi, the causative agent of Lyme disease, undergoes a loss in virulence with repeated passage in vitro. Defining the changes which occur after conversion to avirulence may assist in identifying virulence factors and mechanisms of pathogenesis. We have used a cross-adsorption technique and two-dimensional nonequilibrium pH gradient electrophoresis to compare virulent (low-passage) and avirulent (high-passage) variants of B. Burgdorferi B31. Using cross-adsorbed rabbit sera to probe immunoblots, we identified 10 low-passage-associated proteins (relative molecular masses of 78, 58, 49, 34, 33, 28, 24, 20, and 16 kDa) unique to the virulent strain B31. Cross-adsorbed human serum detected five proteins of similar sizes (78, 58, 34, 28, and 20 kDa), suggesting that several of of these proteins were expressed during human infection. By probing inner and outer membranes, two proteins (58 and 33 kDa) that localized specifically to the outer membrane were observed. An additional low-passage-associated protein (28 kDa) was identified when outer membranes from low- and high-pressure variants of strain B31 were compared by two-dimensional nonequilibrium pH gradient electrophoresis.