Effect of long-term confinement on metabolic and physiological parameters in mice.
Effect of long-term confinement on metabolic and physiological parameters in mice.
复制标题
长期限制对小鼠代谢和生理参数的影响。
DOI：
10.1016/j.physbeh.2021.113386
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发表时间：
2021
影响因子：
2.9
通讯作者：
Noda Y.
中科院分区：
文献类型：
--
作者：
Nishimura W;Takayanagi Y;Tumurkhuu M;Zhou R;Miki H;Noda Y.
在封闭环境中的长期和轻度禁闭或隔离可能发生在灾害，特定的政治，经济或社会事件，核避难所，海底勘探，极地探险和太空旅行等情况下。为了研究长期封闭环境对哺乳动物应激的影响，我们将8周龄C57 BL/6 J小鼠分为4组，分别饲养在封闭环境中，其中包括狭窄代谢笼（应激组）、正常代谢笼（对照组）、常规笼（常规组）和带金属丝网地板的常规笼（金属丝网组）。对小鼠的表型进行了为期四周的检查，随后进行了行为测试。在应激组中观察到体重增加抑制。使用植入的测量装置对这些小鼠每两分钟进行一次连续分析，持续四周，结果显示应激组的自发活动量和皮下温度显著降低。在每种环境中生活四周后，应激组小鼠的行为测试也显示，在转棒测试中，跌倒的潜伏期较短，在足迹测试中，步幅和步间距较短。有趣的是，应激组小鼠较低的自发活动通过饲养在常规笼中得以挽救。这些结果表明，在一个封闭的环境中长期禁闭作为一种慢性和温和的压力对哺乳动物的稳态的暂时影响。
Long-term and mild confinement or isolation in an enclosed environment can occur in situations such as disasters, specific political, economic or social events, nuclear shelters, seabed exploration, polar expeditions, and space travel. To investigate the effects of stress caused by long-term confinement in an enclosed environment in mammals, we divided 8-week-old C57BL/6J mice into four groups that were housed in a closed environment with a narrow metabolic cage (stress group), normal metabolic cage (control group), conventional cage (conventional group) or conventional cage with wire mesh floor (wire mesh group). The phenotypes of the mice were examined for four weeks, followed by behavioral tests. Weight gain suppression was observed in the stress group. Continuous analysis of these mice every two minutes for four weeks using an implanted measuring device showed a significantly decreased amount of spontaneous activity and subcutaneous temperature in the stress group. After housing in each environment for four weeks, the behavioral tests of mice in the stress group also revealed a shorter latency to fall off in the rotarod test and shorter stride length and interstep distance in the footprint test. Interestingly, the lower spontaneous activity of mice in the stress group was rescued by housing in conventional cages. These results suggest a temporary effect of long-term confinement in an enclosed environment as a chronic and mild stress on homeostasis in mammals.