Cytochromes P-450 of sea birds: cross-reactivity studies with purified rat cytochromes.
Cytochromes P-450 of sea birds: cross-reactivity studies with purified rat cytochromes.
复制标题
海鸟细胞色素 P-450：与纯化的大鼠细胞色素的交叉反应性研究。
DOI：
10.3109/00498258909043169
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Colin H. Walker
中科院分区：
文献类型：
--
作者：
M. J. J. Ronis;T. Hansson;J. Borlakoglu;Colin H. Walker
1.用抗大鼠细胞色素P-450c(IA1)、P-450d(IA2)、P-450b(IIB1)、P-450h(IIC11)和P-450j(IIE1)的多克隆抗体，对1978-1988年在爱尔兰海域采集的6种海鸟的肝微粒体进行了检测。2.在所有种类的海鸟中，仅与P450IA1抗体有显著的交叉反应。交叉反应蛋白在不同禽类之间的表达差异很大，这表明环境诱导的证据。3.P450IA1和IA2的共同表位存在于一种单一的蛋白上，该蛋白表达于喙(Phalacrocorax Caro)肝微粒体中。4.抗大鼠P450基因家族II成员的抗体与海鸟微粒体有少量的交叉反应。抗P450 IIB1和IIC11的抗体在所有物种中表现出弱的交叉反应，个体间差异很小。抗P450 IIE1的抗体在任何鸟类中均无交叉反应。5.P450基因家族I在海鸟中有较好的表达，而P450基因家族II在这类低等脊椎动物中不太发达。
1. Polyclonal antibodies against rat cytochrome P-450c (IA1), P-450d (IA2), P-450b (IIB1), P-450h (IIC11) and P-450j (IIE1), were used to probe liver microsomes prepared from six sea bird species collected from the Irish Sea between 1978 and 1988. 2. Significant cross-reactivity in all the sea bird species was seen only with antibodies to P450 IA1. Expression of cross-reactivity proteins was highly variable between individual birds, which show evidence of environmental induction. 3. Shared epitopes to P450 IA1 and IA2 were seen on a single protein expressed in liver microsomes from the cormorant (Phalacrocorax carbo). 4. Antibodies against members of rat P450 gene family II showed a small degree of cross-reactivity with sea bird microsomes. Antibodies against P450 IIB1 and IIC11 showed weak cross-reactivity in all species with little inter-individual variation. Antibodies to P450 IIE1 showed no cross-reactivity in any bird species. 5. P450 gene family I appears to be well represented in sea birds while P450 gene family II is not well developed in this group of lower vertebrates.
由来自β-萘黄酮喂养的虹鳟鱼的细胞色素 P-450 的纯化同工酶催化的苯并[a]芘羟化酶。
影响因子：
5.8
作者：
Williams,DE;Buhler,DR
通讯作者：
Buhler,DR