The early lethality of autosomal monosomy in the mouse.
The early lethality of autosomal monosomy in the mouse.
复制标题
小鼠常染色体单体的早期致死率。
DOI：
10.1002/jez.1402360313
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Epstein,CJ
中科院分区：
文献类型：
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作者：
Magnuson,T;Debrot,S;Dimpfl,J;Zweig,A;Zamora,T;Epstein,CJ
使用雄性小鼠双杂合的罗伯逊易位染色体对，有一个手臂在共同的，小鼠胚胎单体的19个常染色体中的11个已经产生。所有这些单体导致在植入期之前或期间死亡，只有极少数存活者在受精后6天被检测到。对于一些单体，在发育的第三天或第四天可以检测到致死性的开始，但是其他单体直到第4天的晚期囊胚阶段之后的某个时间才开始死亡。发育迟缓，如细胞数量减少所揭示的，通常在致死期开始之前或之后可检测到。死亡发生的时期可能会持续几天，并且与着床前或围着床期的任何发育标志不一致。可能影响细胞增殖速率或胚胎发育其他方面的遗传因素似乎在确定个体单体性导致死亡的确切时间方面发挥着重要作用。常染色体单体的普遍早期致死性导致了这样的结论，即分散在所有常染色体上的大量基因座参与了浓度依赖性很强的过程，以至于50%的减少足以产生非常严重的后果。
Using male mice doubly heterozygous for pairs of Robertsonian translocation chromosomes that have one arm in common, mouse embryos monosomic for 11 of the 19 autosomes have been generated. All of these monosomies result in death prior to or during the implantation period, with only rare survivors being detected 6 days after fertilization. For some of the monosomies the onset of lethality can be detected during the third or fourth day of development, but others do not begin to die until sometime after the late blastocyst stage on day 4. Retardation of development, as revealed by decreased cell numbers, is often detectable prior to or after the onset of the lethal period. The period during which death occurs may spread over several days and does not coincide with any of the developmental landmarks of the pre‐ or peri‐implantation period. Genetic factors that may affect the rate of cellular proliferation or other aspects of embryonic development appear to play an important role in determining exactly when individual monosomies result in death. The universal early lethality of the autosomal monosomies leads to the conclusion that a large number of loci scattered over all of the autosomes are involved in processes that are so concentration dependent that a 50% reduction is sufficient to produce very serious consequences.