Molecular analyses of tomato GS, GOGAT and GDH gene families and their response to abiotic stresses
Molecular analyses of tomato GS, GOGAT and GDH gene families and their response to abiotic stresses
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番茄 GS、GOGAT 和 GDH 基因家族的分子分析及其对非生物胁迫的响应
DOI：
10.1007/s11738-016-2251-2
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发表时间：
2016-09-01
影响因子：
2.6
通讯作者：
Lu, Yongen
中科院分区：
文献类型：
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作者：
Liu, Lifeng;Wang, Jing;Lu, Yongen
谷氨酰胺合成酶（GS）、谷氨酸合成酶（GOGAT）和谷氨酸脱氢酶（GDH）是植物氮代谢中密切相关的酶，也是提高氮素利用效率的潜在靶标。然而，很少有研究集中在番茄中的酶编码基因。在这里，对这些基因进行了全面的研究。在番茄中鉴定出6个GS基因、2个GOGAT基因和5个GDH基因。SlNADH-GOGAT、SlGS 1. 1和SlNAD-GDHB 1在番茄根中大量表达，SlGS 1. 1可被氮剥夺诱导表达，SlGS 1.2、SlGS 1.3、SlNADH-GOGAT和SlNAD-GDHB 1可被氮剥夺5 d后的再供应诱导表达，SlFd-GOGAT、SlGS 1. 1和SlNAD-GDHA 1-A2在果实中也有高表达，表明它们在果实发育和成熟过程中起重要作用。番茄GS、GOGAT和GDH基因在干旱、低温或高温胁迫下表达水平发生变化，可能参与了番茄的逆境响应。这些研究结果将有助于探索这些基因的生物学功能和调控机制，以及提高番茄氮素利用效率、抗逆性和果实品质的研究。
Glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) are closely related enzymes in plant nitrogen metabolism and potential targets for improving nitrogen use efficiency. However, little research has focused on the enzyme-encoding genes in tomato. Here, a comprehensive study of these genes was conducted. Six GS genes, two GOGAT genes and five GDH genes were identified in tomato. Bioinformatics and gene expression analyses suggested that these genes evolved species-specific regulatory properties and biological functions in tomato.SlNADH-GOGAT,SlGS1.1andSlNAD-GDHB1were abundantly expressed in roots,SlGS1.1can be induced by nitrogen deprivation, andSlGS1.2,SlGS1.3,SlNADH-GOGATandSlNAD-GDHB1can be induced by the re-supply of nitrogen after 5 days of deprivation, they may play key roles in primary nitrogen assimilation.SlFd-GOGAT,SlGS1.1andSlNAD-GDHA1-A2were also highly expressed in fruits, indicating their important roles in fruit development and ripening. Tomato GS, GOGAT and GDH may be involved in stress responsiveness, since most of these genes modified their expression levels under drought, cold or heat stress treatment. We believe these findings will assist in the exploration of the genes’ biological functions and regulatory mechanisms, as well as the studies to improve nitrogen use efficiency, stress resistance and fruit quality in tomato.