Structure of (dG)n.(dC)n under superhelical stress and acid pH.
Structure of (dG)n.(dC)n under superhelical stress and acid pH.
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超螺旋应力和酸性 pH 条件下 (dG)n.(dC)n 的结构。
DOI：
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发表时间：
1987
影响因子：
4.4
通讯作者：
M. Frank
中科院分区：
文献类型：
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作者：
V. Lyamichev;S. Mirkin;M. Frank
我们最近发现，(GA)n.(TC)n轨道在超螺旋应力下经历了急剧的结构转变(VI.Lyamichev，S.M.Mirkin和M.D.Frank-Kamenetskii，J.Biol)。结构。戴恩。2327(1985)。与研究得很好的十字形和Z型转变不同，这种新的转变强烈地依赖于pH。我们发现(DG)n.(DC)n插入物经历了与(GA)n.(TC)n区类似的依赖于pH的结构转变。这些新数据符合我们早先的预期，与D.E.Pulley Blank、D.B.Haniford和A.R.Morgan，Cell 42,271(1985)的数据不一致。我们得出结论，在超螺旋压力和/或酸性pH下，一种新的DNA结构(H型)是典型的高嘌呤-高嘧啶镜像重复序列(H回文)。在H型中，高嘧啶链形成一个发夹，而一半的高嘌呤链与发夹相互作用形成三链，另一半的高嘌呤链是非结构化的(V.I.Lyamichev，S.M.Mirkin和M.D.Frank-Kamenetskii，J.biol)。结构。戴恩。2,3667(1986)。
We have recently shown that a (GA)n.(TC)n tract undergoes a sharp structural transition under superhelical stress (V.I. Lyamichev, S.M. Mirkin and M.D. Frank-Kamenetskii, J. Biomol. Struct. Dyn. 2,327 (1985]. Unlike the well studied transitions to the cruciform and to the Z form, this novel transition was strongly pH-dependent. We have found the (dG)n.(dC)n insert to undergo a pH-dependent structural transition similar to that of the (GA)n.(TC)n tract. These new data meet our earlier expectations and disagree with the data of D.E. Pulleyblank, D.B. Haniford and A.R. Morgan, Cell 42, 271 (1985). We conclude that a novel DNA structure (the H-form) is typical of homopurine-homopyrimidine mirror repeats (the H palindromes) under superhelical stress and/or acid pH. In the H-form the homopyrimidine strand forms a hairpin while half of the homopurine strand interacts with the hairpin forming a triplex, the other half of the homopurine strand being unstructured (V.I. Lyamichev, S.M. Mirkin and M.D. Frank-Kamenetskii, J. Biomol. Struct. Dyn. 2, 3, 667 (1986].
影响因子：
14.9
作者：
Shen,CK
通讯作者：
Shen,CK
人类 U1 RNA 基因在保守的 3 侧翼区域内含有异常敏感的核酸酶 S1 切割位点。
DOI：
10.1073/pnas.81.23.7288
发表时间：
1984
影响因子：
11.1
作者：
Htun,H;Lund,E;Dahlberg,JE
通讯作者：
Dahlberg,JE