Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months
Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months
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DOI：
10.1073/pnas.1701425114
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发表时间：
2017-06-06
影响因子：
11.1
通讯作者：
Wakayama, Teruhiko
中科院分区：
文献类型：
--
作者：
Wakayama, Sayaka;Kamada, Yuko;Wakayama, Teruhiko
如果人类开始在太空中永久生活，使用保存精子的辅助生殖技术对于生育后代将是重要的；然而，国际空间站（ISS）上的辐射比地球上强100倍以上，辐射会导致细胞和配子中的DNA损伤。在这里，我们研究了空间辐射对冷冻干燥的小鼠精子的影响，这些精子在国际空间站上保存了9个月，温度为-95摄氏度，在室温下发射和回收。精子和雄性原核的DNA损伤略有增加，但受精率和出生率与对照组相似。下一代测序显示，空间保存精子的后代和对照组之间只有很小的基因组差异，所有的后代都长大成人，具有正常的生育能力。因此，我们证明，尽管空间辐射会损害精子DNA，但在国际空间站上储存至少9个月后，它不会影响可存活后代的产生。
If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at -95 degrees C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls. Next-generation sequencing showed only minor genomic differences between offspring derived from space-preserved spermatozoa and controls, and all offspring grew to adulthood and had normal fertility. Thus, we demonstrate that although space radiation can damage sperm DNA, it does not affect the production of viable offspring after at least 9 mo of storage on the ISS.