Fermentative valorisation of xylose-rich hemicellulosic hydrolysates from agricultural waste residues for lactic acid production under non-sterile conditions.
Fermentative valorisation of xylose-rich hemicellulosic hydrolysates from agricultural waste residues for lactic acid production under non-sterile conditions.
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DOI：
10.1016/j.wasman.2023.05.015
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发表时间：
2023-05
期刊：
影响因子：
8.1
通讯作者：
Rylan Cox;Vivek Narisetty;E. Castro;Deepti Agrawal;S. Jacob;Gopalakrishnan Kumar;Deepak Kumar;Vinod Kumar
中科院分区：
文献类型：
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作者：
Rylan Cox;Vivek Narisetty;E. Castro;Deepti Agrawal;S. Jacob;Gopalakrishnan Kumar;Deepak Kumar;Vinod Kumar
乳酸（LA）是一种具有多种工业用途的平台化学品。目前，LA的商业化生产主要是利用含糖或淀粉基原料进行微生物发酵。研究追求强调使用不可食用和可再生原料可持续生产LA，加速了木质纤维素生物质（LCB）的使用。以甘蔗渣（SCB）和橄榄核（OP）为原料，分别采用水热法和稀酸法对木糖进行预处理。获得的富含木糖的水解产物在非无菌条件下由同质发酵和嗜热的凝固芽孢杆菌dsm2314菌株生产LA。纯木糖、富含木糖的SCB和OP水解产物的LA滴度最高分别为97.8、52.4和61.3 g/L，产率分别为0.77、0.66和0.71 g/g。采用两步水两相系统（ATPS）萃取技术分离和回收木糖上积累的LA。第一步LA回收率为45 ~ 65%，第二步提高到80 ~ 90%。该研究展示了一种高效的综合生物精炼方法，可以提高富含木糖的废水的成本效益，从而生产和回收LA。
Lactic acid (LA) is a platform chemical with diverse industrial applications. Presently, commercial production of LA is dominated by microbial fermentation using sugary or starch-based feedstocks. Research pursuits emphasizing towards sustainable production of LA using non-edible and renewable feedstocks have accelerated the use of lignocellulosic biomass (LCB). The present study focuses on the valorisation of xylose derived from sugarcane bagasse (SCB) and olive pits (OP) through hydrothermal and dilute acid pretreatment, respectively. The xylose-rich hydrolysate obtained was used for LA production by homo-fermentative and thermophilicBacillus coagulansDSM2314 strain under non-sterile conditions. The fed-batch mode of fermentation resulted in maximum LA titers of 97.8, 52.4 and 61.3 g/L with a yield of 0.77, 0.66 and 0.71 g/g using pure xylose, xylose-rich SCB and OP hydrolysates, respectively. Further, a two-step aqueous two-phase system (ATPS) extraction technique was employed for the separation and recovery of LA accumulated on pure and crude xylose. The LA recovery was 45 – 65% in the first step and enhanced to 80–90% in the second step.The study demonstrated an efficient integrated biorefinery approach to valorising the xylose-rich stream for cost-effective LA production and recovery.