1H, 13C, and 15N resonance assignments of the dsRBDs of mouse RNA helicase A
1H, 13C, and 15N resonance assignments of the dsRBDs of mouse RNA helicase A
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小鼠 RNA 解旋酶 A dsRBD 的 1H、13C 和 15N 共振分配
DOI：
10.1007/s12104-012-9380-3
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and Yutaka Muto
中科院分区：
文献类型：
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作者：
Takashi Nagata T;Kengo Tsuda;Naohiro Kobayashi;Peter Guntert;Shigeyuki Yokoyama;and Yutaka Muto
RNA解旋酶A（RHA）是一种多功能的基因表达调控蛋白。RHA具有两个双链RNA结合结构域（dsRBD），其作为高度结构化的RNA结合和蛋白质-蛋白质相互作用的模块。使用dsRBD，RHA结合细胞和病毒mRNA，将它们从细胞核输出，并调节剪接以及翻译起始。据报道，RHA dsRBD还介导与小RNA和其他含dsRBD的蛋白质的相互作用，并共同形成参与RNA沉默途径的加工复合物。此外，RHA dsRBD将RNA聚合酶II与几种转录因子桥接。本文报道了RHA的dsRBD的~ 1H、~（13）C和~（15）N化学位移归属。在这项工作中获得的共振分配将有助于阐明RHA和转录或转录后基因调控之间的相互作用。
RNA helicase A (RHA) is a multifunctional protein that regulates gene expression. RHA has two double-stranded RNA-binding domains (dsRBDs) that serve as modules for highly structured RNA binding and protein–protein interactions. Using the dsRBDs, RHA binds to cellular and viral mRNAs, exports them from the nucleus, and regulates splicing as well as translational initiation. The RHA dsRBDs also reportedly mediate interactions with small RNAs and other dsRBD-containing proteins, and altogether form a processing complex involved in RNA silencing pathways. In addition, the RHA dsRBDs bridge RNA polymerase II with several transcription factors. Here we report the1H,13C, and15N chemical shift assignments of the dsRBDs of RHA. The resonance assignments obtained in this work will contribute to the elucidation of the interactions between RHA and transcriptional or post-transcriptional gene regulators.