Topical application of mitomycin C prevents epineural scar formation in rats
Topical application of mitomycin C prevents epineural scar formation in rats
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DOI：
10.1007/s10143-004-0370-5
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发表时间：
2005-04-01
影响因子：
2.8
通讯作者：
Ceylan, S
中科院分区：
文献类型：
--
作者：
Ilbay, K;Etus, V;Ceylan, S
通过大体解剖和组织学分析，观察局部应用丝裂霉素C对大鼠术后神经周纤维化的预防作用。24只成年雄性Wistar大鼠双侧暴露坐骨神经，所有动物股二头肌裸露表面均产生磨损损伤。实验组神经周围放置丝裂霉素C(0.5 mg/ml)浸泡棉垫5min，对照组放置生理盐水浸泡棉垫5min。术后4周，采用盲法手术解剖评价神经松解部位。神经周围粘连采用数值分级方案进行分级。计算瘢痕组织形成指数，并根据组织学评价中神经外膜周围成纤维细胞/成纤维细胞数进行分级。经丝裂霉素C处理的神经周围粘连明显少于对照组。对神经周围致密结缔组织的定量显示，丝裂霉素C治疗后神经周围的密度显著减少，成纤维细胞/纤维细胞的数量也显著减少。局部应用丝裂霉素C可能有效地预防周围神经松解后神经外膜瘢痕的形成。
The role of topically applied mitomycin C in preventing postoperative perineural fibrosis was examined by gross anatomical dissection and histological analysis in rats. The sciatic nerve was exposed bilaterally in 24 Wistar adult male rats, and an abrasion injury was produced on the exposed surface of the biceps femoris muscle in all animals. In the experimental group, cotton pads soaked with mitomycin C (0.5 mg/ml) were placed around the nerves for 5 min, whereas cotton pads soaked with saline were applied to the control group. Four weeks after surgery, the neurolysis sites were evaluated by blinded surgical dissection. Perineural adhesions were graded using a numerical grading scheme. The scar tissue formation index was also calculated, and a grading was made according to the number of fibroblasts/fibrocytes counted around the epineurium in histological evaluation. Mitomycin C-treated nerves showed significantly less perineural adhesions than controls. Quantification of the dense connective tissue surrounding the nerves revealed a statistically significant reduction around nerves treated with mitomycin C, and the number of fibroblast/fibrocytes was also significantly reduced. Application of topical mitomycin C might be effective in preventing epineural scar formation after neurolysis of peripheral nerves.