Molecular characterization of nine sweet potato (Ipomoea batatas Lam.) MADS-box transcription factors during storage root development and following abiotic stress
Molecular characterization of nine sweet potato (Ipomoea batatas Lam.) MADS-box transcription factors during storage root development and following abiotic stress
复制标题
九种甘薯 (Ipomoea batatas Lam.) MADS-box 转录因子在贮藏根发育和非生物胁迫后的分子特征
DOI：
10.1111/pbr.12613
复制
发表时间：
2018-10-01
期刊：
影响因子：
2
通讯作者：
Li, ZongYun
中科院分区：
文献类型：
--
作者：
Dong, TingTing;Song, WeiHan;Li, ZongYun
甘薯是研究贮藏根发育和抗性的重要植物。MADS-box蛋白是一类重要的转录因子，在植物的生长发育和逆境应答中起着重要的作用。然而，迄今为止，对甘薯MADS盒基因的研究仍然有限。本研究克隆了9个MADS-box基因，命名为IbMBP 1-IbMBP 9。9个基因表现出不同的组织特异性表达谱。IbMBP 2、IbMBP 3、IbMBP 4和IbMBP 9在贮藏根发育过程中表达量较高，并受贮藏根发育相关激素的上调。不同程度的诱导下观察到这些MADS盒基因的转录水平在多个非生物胁迫，以及应力相关的激素。结果表明，9个MADS-box基因在甘薯中具有不同的组织特异性表达谱，并受到多种非生物胁迫和激素的影响，为进一步研究MADS-box基因在甘薯中的功能奠定了基础。
Sweet potato is an important plant for researchers to study storage root development and resistance. MADS-box proteins are a group of important transcription factors which play a crucial role in plant development as well as stress response. However, the investigation regarding sweet potato MADS-box genes remains limited up to now. In this study, nine MADS-box genes, named as IbMBP1-IbMBP9, were cloned and characterized. Nine genes exhibited different tissue-specific expression profiles. IbMBP2, IbMBP3, IbMBP4 and IbMBP9 showed high expression during storage roots development and were upregulated by storage roots development-related hormones. Varying degrees of induction was observed in the transcript level of these MADS-box genes under multiple abiotic stresses, as well as stress-related hormones. The present results revealed that nine MADS-box genes exhibited different tissue-specific expression profiles and were impacted by multiple abiotic stresses and/or hormones, which lay a good foundation for further exploring the function of MADS-box genes in sweet potato.